Chromatographic analysis of allosteric effects between ibuprofen and benzodiazepines on human serum albumin

Jianzhong Chen, I. Fitos, David S. Hage

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

The effects of (R)- and (S)-ibuprofen on the binding of benzodiazepines to human serum albumin (HSA) were examined by biointeraction chromatography. The displacement of benzodiazepines from HSA by (R)- and (S)-ibuprofen was found to involve negative allosteric interactions (or possible direct competition) for most (R)-benzodiazepines. However, (S)-benzodiazepines gave positive or negative allosteric effects and direct competition when displaced by (R)- or (S)-ibuprofen. Association equilibrium constants and coupling constants measured for these effects indicated that they involved two classes of ibuprofen binding regions (i.e., low- and high-affinity sites). Based on these results, a model was proposed to explain the binding of benzodiazepines to HSA and their interactions with ibuprofen. This model gave good agreement with previous reports examining the binding of benzodiazepines to HSA.

Original languageEnglish
Pages (from-to)24-36
Number of pages13
JournalChirality
Volume18
Issue number1
DOIs
Publication statusPublished - 2006

Fingerprint

Chromatographic analysis
Ibuprofen
Benzodiazepines
Serum Albumin
Chromatography
Equilibrium constants

Keywords

  • Allosteric interaction
  • Benzodiazepine
  • Biointeraction chromatography
  • Human serum albumin
  • Ibuprofen

ASJC Scopus subject areas

  • Analytical Chemistry
  • Drug Discovery
  • Organic Chemistry
  • Pharmacology

Cite this

Chromatographic analysis of allosteric effects between ibuprofen and benzodiazepines on human serum albumin. / Chen, Jianzhong; Fitos, I.; Hage, David S.

In: Chirality, Vol. 18, No. 1, 2006, p. 24-36.

Research output: Contribution to journalArticle

@article{f0ee6fa0220447aaa1f7e58c884e4c80,
title = "Chromatographic analysis of allosteric effects between ibuprofen and benzodiazepines on human serum albumin",
abstract = "The effects of (R)- and (S)-ibuprofen on the binding of benzodiazepines to human serum albumin (HSA) were examined by biointeraction chromatography. The displacement of benzodiazepines from HSA by (R)- and (S)-ibuprofen was found to involve negative allosteric interactions (or possible direct competition) for most (R)-benzodiazepines. However, (S)-benzodiazepines gave positive or negative allosteric effects and direct competition when displaced by (R)- or (S)-ibuprofen. Association equilibrium constants and coupling constants measured for these effects indicated that they involved two classes of ibuprofen binding regions (i.e., low- and high-affinity sites). Based on these results, a model was proposed to explain the binding of benzodiazepines to HSA and their interactions with ibuprofen. This model gave good agreement with previous reports examining the binding of benzodiazepines to HSA.",
keywords = "Allosteric interaction, Benzodiazepine, Biointeraction chromatography, Human serum albumin, Ibuprofen",
author = "Jianzhong Chen and I. Fitos and Hage, {David S.}",
year = "2006",
doi = "10.1002/chir.20216",
language = "English",
volume = "18",
pages = "24--36",
journal = "Chirality",
issn = "0899-0042",
publisher = "Wiley-Liss Inc.",
number = "1",

}

TY - JOUR

T1 - Chromatographic analysis of allosteric effects between ibuprofen and benzodiazepines on human serum albumin

AU - Chen, Jianzhong

AU - Fitos, I.

AU - Hage, David S.

PY - 2006

Y1 - 2006

N2 - The effects of (R)- and (S)-ibuprofen on the binding of benzodiazepines to human serum albumin (HSA) were examined by biointeraction chromatography. The displacement of benzodiazepines from HSA by (R)- and (S)-ibuprofen was found to involve negative allosteric interactions (or possible direct competition) for most (R)-benzodiazepines. However, (S)-benzodiazepines gave positive or negative allosteric effects and direct competition when displaced by (R)- or (S)-ibuprofen. Association equilibrium constants and coupling constants measured for these effects indicated that they involved two classes of ibuprofen binding regions (i.e., low- and high-affinity sites). Based on these results, a model was proposed to explain the binding of benzodiazepines to HSA and their interactions with ibuprofen. This model gave good agreement with previous reports examining the binding of benzodiazepines to HSA.

AB - The effects of (R)- and (S)-ibuprofen on the binding of benzodiazepines to human serum albumin (HSA) were examined by biointeraction chromatography. The displacement of benzodiazepines from HSA by (R)- and (S)-ibuprofen was found to involve negative allosteric interactions (or possible direct competition) for most (R)-benzodiazepines. However, (S)-benzodiazepines gave positive or negative allosteric effects and direct competition when displaced by (R)- or (S)-ibuprofen. Association equilibrium constants and coupling constants measured for these effects indicated that they involved two classes of ibuprofen binding regions (i.e., low- and high-affinity sites). Based on these results, a model was proposed to explain the binding of benzodiazepines to HSA and their interactions with ibuprofen. This model gave good agreement with previous reports examining the binding of benzodiazepines to HSA.

KW - Allosteric interaction

KW - Benzodiazepine

KW - Biointeraction chromatography

KW - Human serum albumin

KW - Ibuprofen

UR - http://www.scopus.com/inward/record.url?scp=29744448678&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=29744448678&partnerID=8YFLogxK

U2 - 10.1002/chir.20216

DO - 10.1002/chir.20216

M3 - Article

C2 - 16278829

AN - SCOPUS:29744448678

VL - 18

SP - 24

EP - 36

JO - Chirality

JF - Chirality

SN - 0899-0042

IS - 1

ER -