Chromate tolerance caused by reduced hydroxyl radical production and decreased glutathione reductase activity in Schizosaccharomyces pombe

Zoltán Gazdag, I. Pócsi, J. Belágyi, T. Emri, Ágnes Blaskó, Krisztina Takács, M. Pesti

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

The stable Cr(VI)-tolerant chr1-66T mutant of Schizosaccharomyces pombe, which carries one simple gene mutation responsible for Cr(VI) tolerance, accumulated and reduced the chromate anion (CrO42-) significantly more slowly than did its parental strain 6chr+. The mutant chr1-66T proved to be sensitive to oxidative stressors such as H2O2, menadione, tert-butyl hydroperoxide and Cd2+. Both the Cr(VI) tolerance and the oxidative stress sensitivity were attributed to a decreased specific glutathione reductase activity. These effects were also enhanced with a decrease in the specific mitochondrial Mn-SOD activity.

Original languageEnglish
Pages (from-to)96-103
Number of pages8
JournalJournal of Basic Microbiology
Volume43
Issue number2
DOIs
Publication statusPublished - 2003

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Chromates
Glutathione Reductase
Schizosaccharomyces
Hydroxyl Radical
tert-Butylhydroperoxide
Vitamin K 3
Superoxide Dismutase
Anions
Oxidative Stress
Mutation
Genes
chromium hexavalent ion

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Microbiology
  • Genetics

Cite this

Chromate tolerance caused by reduced hydroxyl radical production and decreased glutathione reductase activity in Schizosaccharomyces pombe. / Gazdag, Zoltán; Pócsi, I.; Belágyi, J.; Emri, T.; Blaskó, Ágnes; Takács, Krisztina; Pesti, M.

In: Journal of Basic Microbiology, Vol. 43, No. 2, 2003, p. 96-103.

Research output: Contribution to journalArticle

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