Chromate sensitivity in fission yeast is caused by increased glutathione reductase activity and peroxide overproduction

Miklós Pesti, Zoltán Gazdag, Tamás Emri, Nelli Farkas, Zsuzsa Koósz, József Belágyi, István Pócsi

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

The Cr(VI)-sensitive mutant chr-51S of the Schizosaccharomyces pombe accumulated chromate (CrO42-) and reduced Cr(V) to much greater extent, than did its parental strain 6 chr+. Sublethal doses of K2Cr2O7 did not induce any adaptive stress response, while H2O2 or menadione pretreatment proved protective against the cell injuries caused by Cr(VI). The intracellular GSH concentration in chr-51S cells was approximately half of that for the 6 chr+. Moreover, the glutathione disulfide reducing capacity of chr-51S was characterized by significantly increased glutathione reductase (GR) and glucose-6-phosphate dehydrogenase activities. These data strongly suggested that, instead of GSH, the NADPH/GR system was the major one-electron Cr(VI) reductant in vivo. The increased Cr(V) reduction in chr-51S mutant was accompanied by high intracellular superoxide and peroxide concentrations, required for formation of the hydroxyl radical (OH). The decreased intracellular GSH levels and the Cr(VI)-sensitive phenotype of the chr-51S cells indicates that GSH might act effectively against chromate by scavenging OH.

Original languageEnglish
Pages (from-to)408-419
Number of pages12
JournalJournal of basic microbiology
Volume42
Issue number6
DOIs
Publication statusPublished - Dec 1 2002

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology

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