Chondrogenic potential of mesenchymal stem cells from patients with rheumatoid arthritis and osteoarthritis

Measurements in a microculture system

Valria Dudics, Aliz Kunstár, J. Kovács, Tamás Lakatos, P. Géher, Béla Gömör, E. Monostori, F. Uher

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

Background: Mesenchymal stem cells (MSCs) have the potential to differentiate into distinct mesenchymal tissues; including cartilage and bone, they can be an attractive cell source for cartilage tissue engineering approaches. Our objective here was to compare the in vitro chondrogenic potential of MSCs isolated from patients with rheumatoid arthritis (RA) and osteoarthritis (OA) with cells from normal donors. Methods: Marrow samples were removed during bone surgery and adherent cell cultures were established. The cells were then passed into a newly developed microaggregate culture system in a medium containing transforming growth factor β3, insulin, dexamethasone and/or demineralized bone matrix. In vitro chondrogenic activity was measured as metabolic sulfate incorporation and type II collagen expression in pellet cultures. Results: Culture-expanded MSCs from RA and OA patients did not differ significantly from the normal population with respect to their chondrogenic potential in vitro. Capability of total protein and proteoglycan synthesis as well as collagen II mRNA expression by cell aggregates was similar for all cell preparations in the presence of the appropriate growth and differentiation factors. Chondroprotective drugs such as chondroitin sulfate and glucosamine enhanced, whereas chloroquine inhibited chondrogenesis in normal donor-derived or patient-derived MSC cultures. Galectin-1, a β-galactoside-binding protein with marked anti-inflammatory activity, stimulated the chondrogenic differentiation of mesenchymal cells in low (

Original languageEnglish
Pages (from-to)307-316
Number of pages10
JournalCells Tissues Organs
Volume189
Issue number5
DOIs
Publication statusPublished - Apr 2009

Fingerprint

Mesenchymal Stromal Cells
Osteoarthritis
Rheumatoid Arthritis
Cartilage
Cell Culture Techniques
Tissue Donors
Galectin 1
Growth Differentiation Factors
Chondrogenesis
Bone and Bones
Galactosides
Bone Matrix
Collagen Type II
Chondroitin Sulfates
Glucosamine
Chloroquine
Transforming Growth Factors
Proteoglycans
Tissue Engineering
Dexamethasone

Keywords

  • Chondrogenesis
  • Mesenchymal stem cells
  • Osteoarthritis
  • Rheumatoid arthritis

ASJC Scopus subject areas

  • Anatomy
  • Histology

Cite this

Chondrogenic potential of mesenchymal stem cells from patients with rheumatoid arthritis and osteoarthritis : Measurements in a microculture system. / Dudics, Valria; Kunstár, Aliz; Kovács, J.; Lakatos, Tamás; Géher, P.; Gömör, Béla; Monostori, E.; Uher, F.

In: Cells Tissues Organs, Vol. 189, No. 5, 04.2009, p. 307-316.

Research output: Contribution to journalArticle

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