Cholinergic innervation of mossy cells in the rat fascia dentata

Thomas Deller, I. Katona, Constantino Cozzari, Michael Frotscher, T. Freund

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

Hilar mossy cells are the main cells of origin of the commissural/associational projection to the inner molecular layer of the rat fascia dentata. In order to analyze the cholinergic innervation of hilar mossy cells, a light and electron microscopic double-labeling technique was used. Immunolabeling for calcitonin gene-related peptide (CGRP) was employed to identify mossy cells and immunocytochemistry for choline acetyltransferase (ChAT) was used to label cholinergic septohippocampal fibers. Cholinergic boutons were abundant around mossy cell somata and on their proximal dendrites. Electron microscopy confirmed that many of these boutons formed synapses with the CGRP-positive mossy cells. These data demonstrate a direct innervation of hilar mossy cells by cholinergic septohippocampal afferents. This connectivity could contribute to the electrophysiological behavior of mossy cells during theta oscillations.

Original languageEnglish
Pages (from-to)314-320
Number of pages7
JournalHippocampus
Volume9
Issue number3
DOIs
Publication statusPublished - 1999

Fingerprint

Dentate Gyrus
Cholinergic Agents
Calcitonin Gene-Related Peptide
Cholinergic Fibers
Choline O-Acetyltransferase
Carisoprodol
Dendrites
Synapses
Electron Microscopy
Immunohistochemistry
Electrons
Light

Keywords

  • Calcitonin gene related peptide
  • Commissural/associational projection
  • Neuropeptide
  • Principal cells
  • Septohippocampal projection
  • Theta rhythm

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Cholinergic innervation of mossy cells in the rat fascia dentata. / Deller, Thomas; Katona, I.; Cozzari, Constantino; Frotscher, Michael; Freund, T.

In: Hippocampus, Vol. 9, No. 3, 1999, p. 314-320.

Research output: Contribution to journalArticle

Deller, Thomas ; Katona, I. ; Cozzari, Constantino ; Frotscher, Michael ; Freund, T. / Cholinergic innervation of mossy cells in the rat fascia dentata. In: Hippocampus. 1999 ; Vol. 9, No. 3. pp. 314-320.
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