Chloroplasts preferentially take up ferric–citrate over iron–nicotianamine complexes in Brassica napus

Brigitta Müller, Krisztina Kovács, Hong Diep Pham, Yusuf Kavak, Jiři Pechoušek, Libor Machala, Radek Zbořil, Kálmán Szenthe, Javier Abadía, F. Fodor, Z. Klencsár, Ádám Solti

Research output: Contribution to journalArticle

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Abstract

Main conclusion: Fe uptake machinery of chloroplasts prefers to utilise Fe(III)–citrate over Fe–nicotianamine complexes. Iron uptake in chloroplasts is a process of prime importance. Although a few members of their iron transport machinery were identified, the substrate preference of the system is still unknown. Intact chloroplasts of oilseed rape (Brassica napus) were purified and subjected to iron uptake studies using natural and artificial iron complexes. Fe–nicotianamine (NA) complexes were characterised by 5 K, 5 T Mössbauer spectrometry. Expression of components of the chloroplast Fe uptake machinery was also studied. Fe(III)–NA contained a minor paramagnetic Fe(II) component (ca. 9%), a paramagnetic Fe(III) component exhibiting dimeric or oligomeric structure (ca. 20%), and a Fe(III) complex, likely being a monomeric structure, which undergoes slow electronic relaxation at 5 K (ca. 61%). Fe(II)–NA contained more than one similar chemical Fe(II) environment with no sign of Fe(III) components. Chloroplasts preferred Fe(III)–citrate compared to Fe(III)–NA and Fe(II)–NA, but also to Fe(III)–EDTA and Fe(III)–o,o′EDDHA, and the Km value was lower for Fe(III)-citrate than for the Fe–NA complexes. Only the uptake of Fe(III)–citrate was light-dependent. Regarding the components of the chloroplast Fe uptake system, only genes of the reduction-based Fe uptake system showed high expression. Chloroplasts more effectively utilize Fe(III)–citrate, but hardly Fe–NA complexes in Fe uptake.

Original languageEnglish
JournalPlanta
DOIs
Publication statusAccepted/In press - Jan 1 2018

Fingerprint

Brassica napus
Chloroplasts
chloroplasts
Citric Acid
citrates
Iron
iron
electronics
spectroscopy
Spectrum Analysis
Light
Genes

Keywords

  • Bathophenanthroline disulfonate
  • Ferric chelate reductase
  • Gene expression
  • Mössbauer spectroscopy
  • Oilseed rape

ASJC Scopus subject areas

  • Genetics
  • Plant Science

Cite this

Müller, B., Kovács, K., Pham, H. D., Kavak, Y., Pechoušek, J., Machala, L., ... Solti, Á. (Accepted/In press). Chloroplasts preferentially take up ferric–citrate over iron–nicotianamine complexes in Brassica napus. Planta. https://doi.org/10.1007/s00425-018-3037-0

Chloroplasts preferentially take up ferric–citrate over iron–nicotianamine complexes in Brassica napus. / Müller, Brigitta; Kovács, Krisztina; Pham, Hong Diep; Kavak, Yusuf; Pechoušek, Jiři; Machala, Libor; Zbořil, Radek; Szenthe, Kálmán; Abadía, Javier; Fodor, F.; Klencsár, Z.; Solti, Ádám.

In: Planta, 01.01.2018.

Research output: Contribution to journalArticle

Müller, B, Kovács, K, Pham, HD, Kavak, Y, Pechoušek, J, Machala, L, Zbořil, R, Szenthe, K, Abadía, J, Fodor, F, Klencsár, Z & Solti, Á 2018, 'Chloroplasts preferentially take up ferric–citrate over iron–nicotianamine complexes in Brassica napus', Planta. https://doi.org/10.1007/s00425-018-3037-0
Müller, Brigitta ; Kovács, Krisztina ; Pham, Hong Diep ; Kavak, Yusuf ; Pechoušek, Jiři ; Machala, Libor ; Zbořil, Radek ; Szenthe, Kálmán ; Abadía, Javier ; Fodor, F. ; Klencsár, Z. ; Solti, Ádám. / Chloroplasts preferentially take up ferric–citrate over iron–nicotianamine complexes in Brassica napus. In: Planta. 2018.
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AU - Kavak, Yusuf

AU - Pechoušek, Jiři

AU - Machala, Libor

AU - Zbořil, Radek

AU - Szenthe, Kálmán

AU - Abadía, Javier

AU - Fodor, F.

AU - Klencsár, Z.

AU - Solti, Ádám

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