Chlorophyll b deficiency in soybean mutants. II. Thylakoid membrane development and differentiation

M. Droppa, M. L. Ghirardi, G. Horváth, A. Melis

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

The expression of chlorophyll (Chl) b deficiency in the y9y9 virescent soybean mutant depends strongly on the light intensity during plant growth. The mutant phenotype (high Chl a Chl b ratios, low chlorophyll content per leaf area and low chlorophyll to carotenoid ratios) is expressed at moderate to high intensities, whereas at low light intensities mutant and wild-type plants are indistinguishable. Expression of the mutation appears to be developmentally controlled and is manifested at the membrane level by inhibition of assembly/incorporation of the peripheral complement of the Chl a b light-harvesting complex II (LHC II-peripheral). Analysis of thylakoid membrane polypeptides by SDS-polyacrylamide gel electrophoresis revealed the presence of novel polypeptides of 12-15 kDa in the y9y9 mutants. These polypeptides were the most abundant protein in the thylakoid membrane when the mutation was fully expressed ( Chl a Chl b = 8) but were largely absent both from the mature wild-type and from the fully developed y9y9 chloroplasts ( Chl a Chl b = 3.5). The relative concentration of polypeptides in the 12-15 kDa region was inversely proportional to that of LHC II, suggesting a role for these novel proteins in the assembly of the LHC II and/or chloroplast development.

Original languageEnglish
Pages (from-to)138-145
Number of pages8
JournalBBA - Bioenergetics
Volume932
Issue numberC
DOIs
Publication statusPublished - 1988

Fingerprint

Thylakoids
Soybeans
Membranes
Peptides
Chlorophyll
Chloroplasts
Light
Thylakoid Membrane Proteins
Carotenoids
Electrophoresis
Mutation
Proteins
Polyacrylamide Gel Electrophoresis
chlorophyll b
chlorophyll a
Phenotype
Growth

Keywords

  • (Soybean chloroplast)
  • Chlorophyll b deficiency
  • Light harvesting complex II assembly
  • Photosynthetic unit size
  • Thylakoid membrane polypeptide

ASJC Scopus subject areas

  • Biophysics

Cite this

Chlorophyll b deficiency in soybean mutants. II. Thylakoid membrane development and differentiation. / Droppa, M.; Ghirardi, M. L.; Horváth, G.; Melis, A.

In: BBA - Bioenergetics, Vol. 932, No. C, 1988, p. 138-145.

Research output: Contribution to journalArticle

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AU - Ghirardi, M. L.

AU - Horváth, G.

AU - Melis, A.

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AB - The expression of chlorophyll (Chl) b deficiency in the y9y9 virescent soybean mutant depends strongly on the light intensity during plant growth. The mutant phenotype (high Chl a Chl b ratios, low chlorophyll content per leaf area and low chlorophyll to carotenoid ratios) is expressed at moderate to high intensities, whereas at low light intensities mutant and wild-type plants are indistinguishable. Expression of the mutation appears to be developmentally controlled and is manifested at the membrane level by inhibition of assembly/incorporation of the peripheral complement of the Chl a b light-harvesting complex II (LHC II-peripheral). Analysis of thylakoid membrane polypeptides by SDS-polyacrylamide gel electrophoresis revealed the presence of novel polypeptides of 12-15 kDa in the y9y9 mutants. These polypeptides were the most abundant protein in the thylakoid membrane when the mutation was fully expressed ( Chl a Chl b = 8) but were largely absent both from the mature wild-type and from the fully developed y9y9 chloroplasts ( Chl a Chl b = 3.5). The relative concentration of polypeptides in the 12-15 kDa region was inversely proportional to that of LHC II, suggesting a role for these novel proteins in the assembly of the LHC II and/or chloroplast development.

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