Chlorophyll a fluorescence

Beyond the limits of the QA model

G. Schansker, Szilvia Z. Tóth, Alfred R. Holzwarth, G. Garab

Research output: Contribution to journalArticle

68 Citations (Scopus)

Abstract

Chlorophyll a fluorescence is a non-invasive tool widely used in photosynthesis research. According to the dominant interpretation, based on the model proposed by Duysens and Sweers (1963, Special Issue of Plant and Cell Physiology, pp 353-372), the fluorescence changes reflect primarily changes in the redox state of QA, the primary quinone electron acceptor of photosystem II (PSII). While it is clearly successful in monitoring the photochemical activity of PSII, a number of important observations cannot be explained within the framework of this simple model. Alternative interpretations have been proposed but were not supported satisfactorily by experimental data. In this review we concentrate on the processes determining the fluorescence rise on a dark-to-light transition and critically analyze the experimental data and the existing models. Recent experiments have provided additional evidence for the involvement of a second process influencing the fluorescence rise once QA is reduced. These observations are best explained by a light-induced conformational change, the focal point of our review. We also want to emphasize that - based on the presently available experimental findings - conclusions on α/ß-centers, PSII connectivity, and the assignment of FV/FM to the maximum PSII quantum yield may require critical re-evaluations. At the same time, it has to be emphasized that for a deeper understanding of the underlying physical mechanism(s) systematic studies on light-induced changes in the structure and reaction kinetics of the PSII reaction center are required.

Original languageEnglish
Pages (from-to)43-58
Number of pages16
JournalPhotosynthesis Research
Volume120
Issue number1-2
DOIs
Publication statusPublished - 2014

Fingerprint

Photosystem II Protein Complex
photosystem II
Fluorescence
fluorescence
chlorophyll
Light
Plant Physiological Phenomena
cell physiology
Cell Physiological Phenomena
Photosynthesis
reaction kinetics
Physiology
plant physiology
Plant Cells
Quantum yield
quinones
Reaction kinetics
Oxidation-Reduction
concentrates
electrons

Keywords

  • Chl a fluorescence
  • Fluorescence yield changes
  • Light-induced conformational changes
  • OJIP transient
  • Thermal phase

ASJC Scopus subject areas

  • Plant Science
  • Cell Biology
  • Biochemistry
  • Medicine(all)

Cite this

Chlorophyll a fluorescence : Beyond the limits of the QA model. / Schansker, G.; Tóth, Szilvia Z.; Holzwarth, Alfred R.; Garab, G.

In: Photosynthesis Research, Vol. 120, No. 1-2, 2014, p. 43-58.

Research output: Contribution to journalArticle

Schansker, G. ; Tóth, Szilvia Z. ; Holzwarth, Alfred R. ; Garab, G. / Chlorophyll a fluorescence : Beyond the limits of the QA model. In: Photosynthesis Research. 2014 ; Vol. 120, No. 1-2. pp. 43-58.
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