Chlorophyll-a determination with ethanol - A critical test

Éva Pápista, E. Ács, B. Böddi

Research output: Contribution to journalArticle

85 Citations (Scopus)

Abstract

Chlorophyll-a content is widely used as an indicator of the quality of freshwater bodies. Quantification of chlorophyll-a is a routine procedure in the test laboratories of water works, and in research laboratories. Although attempts have been made to standardise the measurement procedure, there are nonetheless many procedures currently in use. This work is focused on a careful re-examination of the ISO: 10260, 1992 standard, which prescribes 90% (v/v) ethanol for chlorophyll extraction and measurement. Chlorophyll contents of cultures of the cyanobacterium Synechococcus elongatus Nägeli and the chlorophyte Scenedesmus acutus Meyen were determined by means of a series of concentrations of ethanol/water mixtures which were employed as extracting agents - the water content was gradually decreased from 20 to 0%. The extraction procedure was verified by measuring the amount of retained water after using both water and oil pumps for filtering the samples. The spectroscopic effects of the presence of water were studied and the molecular background of these spectral phenomena is discussed. The extraction yields obtained with 90% ethanol were compared to those obtained with methanol and acetone. On the basis of the calculated error level, improvements to the ISO: 10260, 1992 standard method have been suggested.

Original languageEnglish
Pages (from-to)191-198
Number of pages8
JournalHydrobiologia
Volume485
DOIs
Publication statusPublished - Oct 1 2002

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ethanol
chlorophyll a
chlorophyll
water
Synechococcus elongatus
testing
water utilities
Scenedesmus
pumps
acetone
Cyanobacteria
methanol
cyanobacterium
pump
water content
oils
test
oil
sampling
methodology

Keywords

  • Algae
  • Chlorophyll-a determination
  • Ethanol
  • ISO standard 10260 (1992)

ASJC Scopus subject areas

  • Aquatic Science

Cite this

Chlorophyll-a determination with ethanol - A critical test. / Pápista, Éva; Ács, E.; Böddi, B.

In: Hydrobiologia, Vol. 485, 01.10.2002, p. 191-198.

Research output: Contribution to journalArticle

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