Chloramphenicol resistance genes in Salmonella enterica subsp. enterica serovar typhimurium isolated from human and animal sources in Hungary

N. Nogrady, I. Gado, P. Zsolt Fekete, J. Pászti

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

The presence of the chloramphenicol resistance genes cat, cmlA, flo, and the role of plasmids and class 1 integrons in the spread and persistence of chloramphenicol resistance were investigated on a collection of 40 Salmonella enterica serovar Typhimurium strains isolated from animals and humans in Hungary, by PCR and conjugation. Three groups of chloramphenicol resistant strains were identified. Eleven animal and 13 human isolates harboured the flo gene, encoding resistance to chloramphenicol and florfenicol, and possessed integrons of 1.0 Kb and 1.2 Kb typically found on the multidrug resistance island of S. Typhimurium DT104. Fifteen human strains had two different chloramphenicol resistance genes: the catB3 gene, identified as a gene cassette within a 1.45 Kb integron, and the catA gene, both of which were located on and transferred by a 140 Kb plasmid from a representative strain to the E. coli recipient via conjugation. A single animal strain had the catA gene alone, which was also transferred by a 35 Kb plasmid via conjugation. These three groups of strains belonged to three distinct genetic clusters, as it was revealed by macrorestriction analysis of 18 selected strains. This study provides information on the versatile genetic background of the chloramphenicol and florfenicol resistances in S. Typhimurium in Hungary and points to the significance of mobile genetic elements such as conjugative R-plasmids and integrons in the spread and persistence of chloramphenicol resistance genes. The results also indicate the predominance of the flo gene among animal strains and its appearance among human strains in Hungary.

Original languageEnglish
Pages (from-to)164-170
Number of pages7
JournalVeterinarni Medicina
Volume50
Issue number4
Publication statusPublished - Apr 2005

Fingerprint

Chloramphenicol Resistance
Salmonella enterica
Hungary
chloramphenicol
Salmonella Typhimurium
Integrons
Genes
animals
genes
florfenicol
plasmids
Plasmids
genetic conjugation
Interspersed Repetitive Sequences
Serogroup
R Factors
multiple drug resistance
Multiple Drug Resistance
Chloramphenicol
Islands

Keywords

  • Cat
  • Chloramphenicol resistance
  • Conjugative R-plasmid
  • Flo
  • S. Typhimurium

ASJC Scopus subject areas

  • veterinary(all)

Cite this

Chloramphenicol resistance genes in Salmonella enterica subsp. enterica serovar typhimurium isolated from human and animal sources in Hungary. / Nogrady, N.; Gado, I.; Zsolt Fekete, P.; Pászti, J.

In: Veterinarni Medicina, Vol. 50, No. 4, 04.2005, p. 164-170.

Research output: Contribution to journalArticle

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