Chiral characterization and quantification of deprenyl-N-oxide and other deprenyl metabolites in rat urine by capillary electrophoresis

É Szöko, T. Tábi, A. S. Halász, M. Pálfi, H. Kalász, K. Magyar

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Chiral capillary electrophoresis has been validated for the quantitative analysis of R-(-)-deprenyl (selegiline) and seven of its metabolites, among them the diastereomeric pair of selegiline-N-oxide in rat urine. Linear calibration curves were obtained over the concentration range: 0.5-100 μM for selegiline, N-desmethylselegiline, methamphetamine, amphetamine, and selegiline-N-oxides, and fram 0.1-100 μM for para-hydraxylated derivatives af N- desmethylselegiline, methamphetamine, and amphetamine. The inter and intra-assay precision and accuracy varied by <15% for all analytes at concentrations of 2.5, 10 and 25 μM, and <20% at the lower limit of quantification (0.5 ar 0.1 μM). The sample extraction procedure was optimised, and sample recoveries ranged: 80-111% and 74-91% at concentrations of 1 and 10 μM, respectively. The extracted urine samples retained quantitative accuracy for at least 5 days after storage at 4°C. The validated method was used for in vivo metabolism studies in rats treated with either single or repeated dose of selegiline, or selegiline-N-oxide. Stereoselective N-oxidation of selegiline and rapid urinary excretion of selegiline-N-oxides have been observed. The most abundant metabolites of selegiline were the desalkylated and parahydoxylated derivatives excreted in both conjugated and unconjugated forms in rat urine.

Original languageEnglish
Pages (from-to)S245-S251
JournalChromatographia
Volume60
Issue numberSUPPL.
Publication statusPublished - Aug 20 2004

Keywords

  • Capillary electrophoresis
  • Deprenyl and deprenyl-N-oxide
  • In vivo metabolism
  • Method validation

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Clinical Biochemistry
  • Organic Chemistry

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