Chiral analysis of amino acid neurotransmitters and neuromodulators in mouse brain by CE-LIF

Tamás Jakó, Eszter Szabó, Tamás Tábi, Gergely Zachar, András Csillag, Éva Szöko

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Chiral CE method has been developed for quantitative determination of D-amino acid modulators of NMDA glutamate receptor, D-serine and D-aspartate along with L-glutamate and L-aspartate in biological samples. These ligands are suggested to be involved in regulation of NMDA receptor related brain functions, such as neurogenesis, neuronal plasticity, and memory formation. For sensitive determination of the amino acids LIF detection was chosen, and a fluorogenic reagent, 7-fluoro-4-nitro-2,1,3-benzoxadiazole was used for derivatization. An amino-modified β-CD, 6-monodeoxy-6-mono(3-hydroxy)propylamino-β-CD (HPA-β-CD) was applied as chiral selector. Determinations were accomplished in a polyacrylamide coated capillary and reverse polarity was used for the analysis of the negatively charged analytes. The method was optimized and validated; 6 mM HPA-β-CD in 50m MHEPES buffer, pH 7 was appropriate to achieve baseline separation of the analytes. The limit of quantification with acceptable accuracy is 0.05 μM for both D-amino acids. Themethod was used for the determination of D-aspartate and D-serine content in various brain regions of adult mice.

Original languageEnglish
Pages (from-to)2870-2876
Number of pages7
JournalELECTROPHORESIS
Volume35
Issue number19
DOIs
Publication statusPublished - Jan 1 2014

Keywords

  • Amino acids
  • CE-LIF
  • Chiral separation
  • NMDA receptor modulators

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Clinical Biochemistry

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