Chimeric genes and transgenic plants are used to study the regulation of genes involved in symbiotic plant‐microbe interactions (nodulin genes)

Frans J. de Bruijn, Laszló Szabados, Jeff Schell

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Nodulin genes are plant genes specifically activated during the formation of nitrogen‐fixing nodules on leguminous plants. These genes are interesting to study since they are not only induced in a specific developmental fashion by signals coming directly or indirectly from the rhizobial symbiont, but are also expressed in a tissue‐specific manner. By examining the expression of chimeric nodulin‐reporter genes in transgenic legume plants it has been shown that nodule specific expression is mediated by DNA sequences present in the 5′upstream region of several nodulin genes. Here we summarize the available data on these cis‐acting elements and the trans‐acting factors interacting with them. We also review experiments designed to identify rhizobial “signals” which may play a role in nodule specific gene expression.

Original languageEnglish
Pages (from-to)182-196
Number of pages15
JournalDevelopmental Genetics
Volume11
Issue number3
DOIs
Publication statusPublished - 1990

Keywords

  • A. rhizogenes
  • Agrobacterium turnefaciens
  • Enod2
  • binary vectors
  • chimeric genes
  • chloramphenicol acetyltransferase
  • cis‐acting elements
  • cytokinin induction
  • glucuronidase
  • glutamine synthetase
  • leghemoglobin
  • nodulins
  • plant transformation
  • transacting factors

ASJC Scopus subject areas

  • Genetics
  • Developmental Biology
  • Cell Biology

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