Chilling sensitivity of carp (cyprinus carpio) embryos at different developmental stages in the presence or absence of cryoprotectants

Work in progress

A. Dinnyés, B. Urbányi, B. Baranyai, I. Magyary

Research output: Contribution to journalArticle

43 Citations (Scopus)

Abstract

The unsolved problem of cryopreservation of the yolk-rich teleost embryos may be related, in part, to their sensitivity to chilling and cryoprotective agents. The aim of this study was to gain data on the sensitivity of carp embryos to low temperatures at different developmental stages and on the possible protective and toxic effects of cryoprotectants. A total of 86,400 morulae, half-epiboly and heartbeat-stage embryos was selected and then placed in water or in 1 M methanol, dimethyl sulfoxide (Me2SO), glycerol or 0.1 M sucrose solution at 0, 4 or 24°C for 5 min or 1 h. Following these treatments, the embryos were held in a 24°C water bath until the evaluation of hatching rates. In every developmental stage a significant decrease of hatching rates following exposure to 4 or 0°C was detected. Sensitivity to chilling changed significantly with development (heartbeat2SO2SO in the heartbeat stage. The results show morulae are partially protected against chilling in Me2SO and sucrose, half-epiboly in Me2SO, sucrose and methanol, and heartbeat-stage in methanol and glycerol. The results further suggest that carp embryos are sensitive to chilling and that toxicity and protective effects against chilling of cryoprotectants are stage-dependent. The finding on the low chilling sensitivity of heartbeat- stage embryos and the protective effect of certain cryoprotectants may be useful in designing cryopreservation protocols.

Original languageEnglish
Pages (from-to)1-13
Number of pages13
JournalTheriogenology
Volume50
Issue number1
DOIs
Publication statusPublished - Jul 1 1998

Fingerprint

Carps
cryoprotectants
Cyprinus carpio
carp
embryo (animal)
Embryonic Structures
developmental stages
Methanol
Sucrose
Morula
methanol
Cryopreservation
morula
sucrose
cryopreservation
Glycerol
protective effect
glycerol
hatching
Cryoprotective Agents

Keywords

  • Carp
  • Chilling sensitivity
  • Cryoprotectant toxicity
  • Embryo
  • Fish

ASJC Scopus subject areas

  • Animal Science and Zoology
  • veterinary(all)

Cite this

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abstract = "The unsolved problem of cryopreservation of the yolk-rich teleost embryos may be related, in part, to their sensitivity to chilling and cryoprotective agents. The aim of this study was to gain data on the sensitivity of carp embryos to low temperatures at different developmental stages and on the possible protective and toxic effects of cryoprotectants. A total of 86,400 morulae, half-epiboly and heartbeat-stage embryos was selected and then placed in water or in 1 M methanol, dimethyl sulfoxide (Me2SO), glycerol or 0.1 M sucrose solution at 0, 4 or 24°C for 5 min or 1 h. Following these treatments, the embryos were held in a 24°C water bath until the evaluation of hatching rates. In every developmental stage a significant decrease of hatching rates following exposure to 4 or 0°C was detected. Sensitivity to chilling changed significantly with development (heartbeat2SO2SO in the heartbeat stage. The results show morulae are partially protected against chilling in Me2SO and sucrose, half-epiboly in Me2SO, sucrose and methanol, and heartbeat-stage in methanol and glycerol. The results further suggest that carp embryos are sensitive to chilling and that toxicity and protective effects against chilling of cryoprotectants are stage-dependent. The finding on the low chilling sensitivity of heartbeat- stage embryos and the protective effect of certain cryoprotectants may be useful in designing cryopreservation protocols.",
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N2 - The unsolved problem of cryopreservation of the yolk-rich teleost embryos may be related, in part, to their sensitivity to chilling and cryoprotective agents. The aim of this study was to gain data on the sensitivity of carp embryos to low temperatures at different developmental stages and on the possible protective and toxic effects of cryoprotectants. A total of 86,400 morulae, half-epiboly and heartbeat-stage embryos was selected and then placed in water or in 1 M methanol, dimethyl sulfoxide (Me2SO), glycerol or 0.1 M sucrose solution at 0, 4 or 24°C for 5 min or 1 h. Following these treatments, the embryos were held in a 24°C water bath until the evaluation of hatching rates. In every developmental stage a significant decrease of hatching rates following exposure to 4 or 0°C was detected. Sensitivity to chilling changed significantly with development (heartbeat2SO2SO in the heartbeat stage. The results show morulae are partially protected against chilling in Me2SO and sucrose, half-epiboly in Me2SO, sucrose and methanol, and heartbeat-stage in methanol and glycerol. The results further suggest that carp embryos are sensitive to chilling and that toxicity and protective effects against chilling of cryoprotectants are stage-dependent. The finding on the low chilling sensitivity of heartbeat- stage embryos and the protective effect of certain cryoprotectants may be useful in designing cryopreservation protocols.

AB - The unsolved problem of cryopreservation of the yolk-rich teleost embryos may be related, in part, to their sensitivity to chilling and cryoprotective agents. The aim of this study was to gain data on the sensitivity of carp embryos to low temperatures at different developmental stages and on the possible protective and toxic effects of cryoprotectants. A total of 86,400 morulae, half-epiboly and heartbeat-stage embryos was selected and then placed in water or in 1 M methanol, dimethyl sulfoxide (Me2SO), glycerol or 0.1 M sucrose solution at 0, 4 or 24°C for 5 min or 1 h. Following these treatments, the embryos were held in a 24°C water bath until the evaluation of hatching rates. In every developmental stage a significant decrease of hatching rates following exposure to 4 or 0°C was detected. Sensitivity to chilling changed significantly with development (heartbeat2SO2SO in the heartbeat stage. The results show morulae are partially protected against chilling in Me2SO and sucrose, half-epiboly in Me2SO, sucrose and methanol, and heartbeat-stage in methanol and glycerol. The results further suggest that carp embryos are sensitive to chilling and that toxicity and protective effects against chilling of cryoprotectants are stage-dependent. The finding on the low chilling sensitivity of heartbeat- stage embryos and the protective effect of certain cryoprotectants may be useful in designing cryopreservation protocols.

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