Chicken or the Egg: Microbial Alterations in Biopsy Samples of Patients with Oral Potentially Malignant Disorders

Gabor Decsi, Jozsef Soki, Bernadett Pap, Gabriella Dobra, Maria Harmati, Sandor Kormondi, Tibor Pankotai, Gabor Braunitzer, Janos Minarovits, Istvan Sonkodi, Edit Urban, Istvan Balazs Nemeth, Katalin Nagy, Krisztina Buzas

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Oral carcinogenesis often leads to the alteration of the microbiota at the site of the tumor, but data are scarce regarding the microbial communities of oral potentially malignant disorders (OPMDs). Punch biopsies were taken from healthy and non-healthy mucosa of OPMD patients to analyze the microbiome using metagenome sequencing. In healthy oral mucosa biopsies the bacterial phyla Firmicutes, Fusobacteria, Proteobacteria, Actinobacteria and Bacteroidetes were detected by Ion Torrent sequencing. The same phyla as well as the phyla Fibrobacteres and Spirochaetes were present in the OPMD biopsies. On the species level, there were 10 bacterial species unique to the healthy tissue and 35 species unique to the OPMD lesions whereas eight species were detected in both samples. We observed that the relative abundance of Streptococcus mitis decreased in the OPMD lesions compared to the uninvolved tissue. In contrast, the relative abundance of Fusobacterium nucleatum, implicated in carcinogenesis, was elevated in OPMD. We detected markedly increased bacterial diversity in the OPMD lesions compared to the healthy oral mucosa. The ratio of S. mitis and F. nucleatum are characteristically altered in the OPMD lesions compared to the healthy mucosa.

Original languageEnglish
Pages (from-to)1023-1033
Number of pages11
JournalPathology and Oncology Research
Volume25
Issue number3
DOIs
Publication statusPublished - Jul 15 2019

Keywords

  • Leukoplakia
  • Lichen
  • Metagenome sequencing
  • OPMD
  • Oral microbiome

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Oncology
  • Cancer Research

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  • Cite this

    Decsi, G., Soki, J., Pap, B., Dobra, G., Harmati, M., Kormondi, S., Pankotai, T., Braunitzer, G., Minarovits, J., Sonkodi, I., Urban, E., Nemeth, I. B., Nagy, K., & Buzas, K. (2019). Chicken or the Egg: Microbial Alterations in Biopsy Samples of Patients with Oral Potentially Malignant Disorders. Pathology and Oncology Research, 25(3), 1023-1033. https://doi.org/10.1007/s12253-018-0457-x