It is generally accepted that keratinocyte migration plays a critical role in the process of wound healing. A study was therefore made of the migratory response of freshly separated and cultured human keratinocytes to factors with chemotactic properties for a variety of cells. Interleukin‐lα (IL‐lα), interferon‐γ (IFN‐γ), interleukin‐8 (IL‐8), and tumour necrosis factor α (TNF‐α) were tested for their chemotactic effectiveness in a modified Boydcn chamber assay. IFN‐γ, IL‐lα and IL‐8 were demonstrated to serve as chemoattractants for freshly separated keratinocytes. For cultured cells, however, only IFN‐γ was found to display chemotactic properties. The findings demonstrate that there is significant difference between the chemotactic behaviour of freshly separated and cultured cells.
|Number of pages||3|
|Journal||Clinical and Experimental Dermatology|
|Publication status||Published - Jul 1994|
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