Chemotactic and stimulating effect of tuftsin and its analogues on human monocytes

M. Kávai, K. Lukács, G. Szegedi, M. Szekerke, J. Érchegyi

Research output: Contribution to journalArticle

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Abstract

Tuftsin, known for its phagocytosis stimulating capacity was examined for its chemotactic activity using a MN cell migration assay based on the Boyden technique. Tuftsin functioned as a chemotactic agent for MN cells in a dose-dependent way with highest activity detected in the concentration range of 50-200 μg/ml tetrapeptide. Pre-incubation of monocytes with tuftsin enhanced locomotion in tests of both random migration and leucotaxis. Structure-activity studies were carried out with [Ala1]-, [Ser1]-, [D-Ser1]-, [Phe3]-, [Phe4]-, [D-Ala4]- and [Glu4]-analogues. Chemotactic activity seemed to be correlated with the presence of the [Thr1]-residue, while the [Phe3]- and [D-Ala4]-analogues exerted significant activity. The stimulation of cell locomotion after pre-incubation of the cells with tuftsin proved less sensitive towards variations in the amino acid sequence. All analogues stimulated chemotaxis, except the [Glu4]-derivative, while random migration was stimulated by [Phe3]-, [Phe4]- and [D-Ser1]-tuftsin. These analogue studies indicated that chemotaxis and random migration can be influenced by different chemical structures.

Original languageEnglish
Pages (from-to)219-224
Number of pages6
JournalImmunology Letters
Volume2
Issue number4
DOIs
Publication statusPublished - 1981

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Tuftsin
Monocytes
Chemotaxis
Cell Migration Assays
Locomotion
Phagocytosis
Cell Movement
Amino Acid Sequence

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy

Cite this

Chemotactic and stimulating effect of tuftsin and its analogues on human monocytes. / Kávai, M.; Lukács, K.; Szegedi, G.; Szekerke, M.; Érchegyi, J.

In: Immunology Letters, Vol. 2, No. 4, 1981, p. 219-224.

Research output: Contribution to journalArticle

Kávai, M. ; Lukács, K. ; Szegedi, G. ; Szekerke, M. ; Érchegyi, J. / Chemotactic and stimulating effect of tuftsin and its analogues on human monocytes. In: Immunology Letters. 1981 ; Vol. 2, No. 4. pp. 219-224.
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