Chemotactic activity of oligopeptides containing an EWS motif on Tetrahymena pyriformis

The effect of amidation of the C-terminal residue

L. Kőhidai, Sz. Bősze, Pál Soós, Eszter Illyés, Orsolya Láng, Marianna Mák, Ferenc Sebestyen, F. Hudecz

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Chemotactic properties of 3-7-mer peptides containing an EWS motive and their peptide amides synthesized and characterized by us were investigated in Tetrahymena pyriformis GL model. Analysis of the peptide acids shows that SEWS possesses exceptionally strong (660% ± 21; 430% ± 18) chemoattractant ability at 10-12 and 10-11 M respectively. The shorter peptide (EWS) possesses chemorepellent activity, while longer peptides display neutral (WSEWS) or moderate chemoattractant (EWSEWS and GEWSEWS) chemotactic ability. Amidation of the C-terminus can significantly modify the character of peptides: it points to the conclusion that a free α-COOH group at this position is required for the high efficiency of SEWS, while in the shorter (EWS) and longer peptides (WSEWS and EWSEWS) amidation can result in chemoattractant ligands. Evaluation of the structure-function relationship of these compounds establishes the significance of Glu (E) with its high surface-exposed area and negatively-charged side chain. The high discriminative ability and good chemotactic responsiveness of Tetrahymena support the theory that a chemotactic signalling mechanism working in higher levels of phylogeny is a well conserved and inducible one even in protozoa.

Original languageEnglish
Pages (from-to)113-120
Number of pages8
JournalCell Biochemistry and Function
Volume21
Issue number2
DOIs
Publication statusPublished - Jun 2003

Fingerprint

Tetrahymena pyriformis
Oligopeptides
Peptides
Chemotactic Factors
Protozoa
Tetrahymena
Phylogeny
Amides
Ligands
Acids

Keywords

  • Chemotaxis
  • Oligopeptides
  • Peptide amides
  • SEWS
  • Signalling
  • Tetrahymena

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Cell Biology

Cite this

Chemotactic activity of oligopeptides containing an EWS motif on Tetrahymena pyriformis : The effect of amidation of the C-terminal residue. / Kőhidai, L.; Bősze, Sz.; Soós, Pál; Illyés, Eszter; Láng, Orsolya; Mák, Marianna; Sebestyen, Ferenc; Hudecz, F.

In: Cell Biochemistry and Function, Vol. 21, No. 2, 06.2003, p. 113-120.

Research output: Contribution to journalArticle

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