Chemosensitizing effect of vitamin C in combination with 5-Fluorouracil in vitro

Beatrix Nagy, Ilona Mucsi, Jozsef Molnar, Andreas Varga, Laszlo Thurzo

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

The antiproliferative effect and apoptosis-inducing action of 5-fluorouracil (5-FU) in combination with vitamin C were tested in vitro against the chemosensitive mouse lymphoma, the chemoresistant HEp-2 and a human lung fibroblast cell line. Vitamin C itself had no antiproliferative effect on the fibroblasts, but increased the anticancer effect of 5-FU dose-dependently. In the case of the chemoresistant cell line, only a high concentration of vitamin C increased the cytotoxicity of 5-FU. A combination of 5-FU and vitamin C exerted a significantly enhanced apoptotic effect on the mouse lymphoma cell line, whereas for the HEp-2 cell line this effect was less marked and was achieved only at a high concentration of vitamin C. These findings suggest that the administration of a high dose of vitamin C in combination with 5-FU chemotherapy enhances the chemoresponsiveness of cancer cells and serves as a potential sensitizer, especially in chemo-resistant cell lines. One of the mechanisms by which vitamin C potentiates cytostatics could be apoptosis induction.

Original languageEnglish
Pages (from-to)289-292
Number of pages4
JournalIn Vivo
Volume17
Issue number3
Publication statusPublished - May 2003

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Fluorouracil
Ascorbic Acid
Cells
Cell Line
Fibroblasts
Lymphoma
Apoptosis
Chemotherapy
Cytostatic Agents
Cytotoxicity
In Vitro Techniques
Drug Therapy
Lung
Neoplasms

Keywords

  • 5-fluorouracil
  • Apoptosis
  • Chemosensitizer
  • Vitamin C

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Chemosensitizing effect of vitamin C in combination with 5-Fluorouracil in vitro. / Nagy, Beatrix; Mucsi, Ilona; Molnar, Jozsef; Varga, Andreas; Thurzo, Laszlo.

In: In Vivo, Vol. 17, No. 3, 05.2003, p. 289-292.

Research output: Contribution to journalArticle

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AB - The antiproliferative effect and apoptosis-inducing action of 5-fluorouracil (5-FU) in combination with vitamin C were tested in vitro against the chemosensitive mouse lymphoma, the chemoresistant HEp-2 and a human lung fibroblast cell line. Vitamin C itself had no antiproliferative effect on the fibroblasts, but increased the anticancer effect of 5-FU dose-dependently. In the case of the chemoresistant cell line, only a high concentration of vitamin C increased the cytotoxicity of 5-FU. A combination of 5-FU and vitamin C exerted a significantly enhanced apoptotic effect on the mouse lymphoma cell line, whereas for the HEp-2 cell line this effect was less marked and was achieved only at a high concentration of vitamin C. These findings suggest that the administration of a high dose of vitamin C in combination with 5-FU chemotherapy enhances the chemoresponsiveness of cancer cells and serves as a potential sensitizer, especially in chemo-resistant cell lines. One of the mechanisms by which vitamin C potentiates cytostatics could be apoptosis induction.

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