Chemically ubiquitylated PCNA as a probe for eukaryotic translesion DNA synthesis

Junjun Chen, Yongxing Ai, Jialiang Wang, Lajos Haracska, Zhihao Zhuang

Research output: Contribution to journalArticle

97 Citations (Scopus)


The rapid growth in ubiquitin biology requires facile chemical approaches for protein ubiquitylation that can overcome the common problem of low yield faced by the enzymatic reaction catalyzed by ubiquitin ligases. We report a chemical approach for monoubiquitylation and SUMOylation of PCNA through disulfide exchange and intein chemistry. We used the chemically ubiquitylated and SUMOylated PCNAs in studying translesion DNA synthesis and revealed a surprising degree of flexibility of the ubiquitin modification.

Original languageEnglish
Pages (from-to)270-272
Number of pages3
JournalNature Chemical Biology
Issue number4
Publication statusPublished - Apr 2010

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

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