Chemically induced cell cycle arrest in perfusion cell culture

Gabor Nagy, Bence Tanczos, Eszter Fidrus, Laszlo Talas, G. Bánfalvi

Research output: Chapter in Book/Report/Conference proceedingChapter

1 Citation (Scopus)

Abstract

In contrast to most present methods, continuous imaging of live cells would require full automation in each processing step. As an integrated system that would meet all requirements does not exist, we have established a long-term scanning-perfusion platform that: (a) replaces old medium with fresh one, (b) bypasses physical contact with the cell culture during continuous cell growth, (c) provides uninterrupted photomicrography of single cells, and (d) secures near physiological conditions and sterility up to several weeks. The system was validated by synchronizing cells using serum starvation and butyrate-induced cell cycle arrest of HaCaT cells.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages161-176
Number of pages16
Volume1524
DOIs
Publication statusPublished - 2017

Publication series

NameMethods in Molecular Biology
Volume1524
ISSN (Print)10643745

Fingerprint

Cell Cycle Checkpoints
Cell Culture Techniques
Perfusion
Photomicrography
Butyrates
Automation
Starvation
Infertility
Growth
Serum

Keywords

  • Cell cycle synchronization
  • Mammalian cell culture
  • Perfusion system
  • Serum starvation
  • Sodium-butyrate synchronization
  • Time-lapse imaging microscopy

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Nagy, G., Tanczos, B., Fidrus, E., Talas, L., & Bánfalvi, G. (2017). Chemically induced cell cycle arrest in perfusion cell culture. In Methods in Molecular Biology (Vol. 1524, pp. 161-176). (Methods in Molecular Biology; Vol. 1524). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-6603-5_10

Chemically induced cell cycle arrest in perfusion cell culture. / Nagy, Gabor; Tanczos, Bence; Fidrus, Eszter; Talas, Laszlo; Bánfalvi, G.

Methods in Molecular Biology. Vol. 1524 Humana Press Inc., 2017. p. 161-176 (Methods in Molecular Biology; Vol. 1524).

Research output: Chapter in Book/Report/Conference proceedingChapter

Nagy, G, Tanczos, B, Fidrus, E, Talas, L & Bánfalvi, G 2017, Chemically induced cell cycle arrest in perfusion cell culture. in Methods in Molecular Biology. vol. 1524, Methods in Molecular Biology, vol. 1524, Humana Press Inc., pp. 161-176. https://doi.org/10.1007/978-1-4939-6603-5_10
Nagy G, Tanczos B, Fidrus E, Talas L, Bánfalvi G. Chemically induced cell cycle arrest in perfusion cell culture. In Methods in Molecular Biology. Vol. 1524. Humana Press Inc. 2017. p. 161-176. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-4939-6603-5_10
Nagy, Gabor ; Tanczos, Bence ; Fidrus, Eszter ; Talas, Laszlo ; Bánfalvi, G. / Chemically induced cell cycle arrest in perfusion cell culture. Methods in Molecular Biology. Vol. 1524 Humana Press Inc., 2017. pp. 161-176 (Methods in Molecular Biology).
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