Chemical fingerprints, antioxidant, enzyme inhibitory, and cell assays of three extracts obtained from Sideritis ozturkii Aytaç & Aksoy

An endemic plant from Turkey

Gokhan Zengin, Asli Uğurlu, Mehmet Cengiz Baloglu, Alina Diuzheva, J. Jekó́, Zoltán Cziáky, Ramazan Ceylan, Abdurrahman Aktumsek, Carene Marie Nancy Picot-Allain, M. Fawzi Mahomoodally

Research output: Contribution to journalArticle

Abstract

This study was geared towards assessing the possible antioxidant, enzyme inhibitory, and cytotoxic activities of ethyl acetate, methanol, and water extracts of Sideritis ozturkii Aytaç & Aksoy. The phytochemical profiles of the studied extracts were characterised by HPLC-MS/MS. The methanol extract, rich in phenolics (78.04 mg gallic acid equivalent/g), exhibited the strongest antioxidant activities. However, the ethyl acetate extract was the most active extract in the enzyme inhibitory assays. The water extract of S. ozturkii (1 mg/ml, 48 h incubation) slightly inhibited (22%) growth of human breast cancer cell line (MDA-MB-231 cells). On the other hand, the ethyl acetate and methanol extracts showed strong inhibition (98% and 97%, respectively) of MDA-MB-231 cells and caused apoptotic cell death. Scientific data generated from this study further appraises the multiple biological activities of plants belonging to the Sideritis genus. In addition, preliminary evidence gathered from the current investigation advocates for further studies geared towards the preparation of therapeutic formulations from S. ozturkii.

Original languageEnglish
Pages (from-to)118-125
Number of pages8
JournalJournal of Pharmaceutical and Biomedical Analysis
Volume171
DOIs
Publication statusPublished - Jul 15 2019

Fingerprint

Sideritis
Dermatoglyphics
Turkey
Methanol
Assays
Antioxidants
Enzymes
Gallic Acid
Water
Enzyme Assays
Phytochemicals
Cell death
Bioactivity
Cell Death
High Pressure Liquid Chromatography
Cells
Breast Neoplasms
Cell Line
Growth
ethyl acetate

Keywords

  • Antioxidant
  • Apoptosis
  • Bioactive agents
  • Diabetes
  • Neuroprotection
  • Sideritis

ASJC Scopus subject areas

  • Analytical Chemistry
  • Pharmaceutical Science
  • Drug Discovery
  • Spectroscopy
  • Clinical Biochemistry

Cite this

Chemical fingerprints, antioxidant, enzyme inhibitory, and cell assays of three extracts obtained from Sideritis ozturkii Aytaç & Aksoy : An endemic plant from Turkey. / Zengin, Gokhan; Uğurlu, Asli; Baloglu, Mehmet Cengiz; Diuzheva, Alina; Jekó́, J.; Cziáky, Zoltán; Ceylan, Ramazan; Aktumsek, Abdurrahman; Picot-Allain, Carene Marie Nancy; Fawzi Mahomoodally, M.

In: Journal of Pharmaceutical and Biomedical Analysis, Vol. 171, 15.07.2019, p. 118-125.

Research output: Contribution to journalArticle

Zengin, Gokhan ; Uğurlu, Asli ; Baloglu, Mehmet Cengiz ; Diuzheva, Alina ; Jekó́, J. ; Cziáky, Zoltán ; Ceylan, Ramazan ; Aktumsek, Abdurrahman ; Picot-Allain, Carene Marie Nancy ; Fawzi Mahomoodally, M. / Chemical fingerprints, antioxidant, enzyme inhibitory, and cell assays of three extracts obtained from Sideritis ozturkii Aytaç & Aksoy : An endemic plant from Turkey. In: Journal of Pharmaceutical and Biomedical Analysis. 2019 ; Vol. 171. pp. 118-125.
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