Amfotericin B tartalmú készítmények kvantitatív analízisének kémiai és mikrobiológiai vonatkozásai

Translated title of the contribution: Chemical and microbiological aspects of the quantitative analysis of amphotericin B

Fittler András, Z. Matus, Kocsis Béla, Botz Lajos

Research output: Contribution to journalArticle

Abstract

Amphotericin B can be determined by chemical (HPLC, spectrophotometry) and microbiological (bioassay) methods. The utilization of both during a stability test can give more detailed information about the activity and concentration change of amphotericin B solutions. Previously published HPLC methods do not lay stress on the separation of by-constituents present in the substance. We have also observed that the bioassay conditions described in the Ph. Eur. 6. are not suitable for the measurement of concentration change experienced during a stability test. The aim of our study was to optimize the chemical and microbiological methods. We have improved the eluent system based on earlier HPLC methods for the separation of the main heptaene and the minor tetraene by-constituents in Fungizone (Bristol-Myers Squibb). The most optimal bioassay conditions were determined where a relatively wide concentration range can be measured. With the improved methods both chemical and microbiological changes can be more accurately measured in our future stability tests.

Original languageHungarian
Pages (from-to)95-102
Number of pages8
JournalActa Pharmaceutica Hungarica
Volume78
Issue number3
Publication statusPublished - 2008

Fingerprint

Amphotericin B
Biological Assay
High Pressure Liquid Chromatography
Spectrophotometry

ASJC Scopus subject areas

  • Pharmaceutical Science

Cite this

Amfotericin B tartalmú készítmények kvantitatív analízisének kémiai és mikrobiológiai vonatkozásai. / András, Fittler; Matus, Z.; Béla, Kocsis; Lajos, Botz.

In: Acta Pharmaceutica Hungarica, Vol. 78, No. 3, 2008, p. 95-102.

Research output: Contribution to journalArticle

@article{3980ed4519c442ffaf334aca61da86f4,
title = "Amfotericin B tartalm{\'u} k{\'e}sz{\'i}tm{\'e}nyek kvantitat{\'i}v anal{\'i}zis{\'e}nek k{\'e}miai {\'e}s mikrobiol{\'o}giai vonatkoz{\'a}sai",
abstract = "Amphotericin B can be determined by chemical (HPLC, spectrophotometry) and microbiological (bioassay) methods. The utilization of both during a stability test can give more detailed information about the activity and concentration change of amphotericin B solutions. Previously published HPLC methods do not lay stress on the separation of by-constituents present in the substance. We have also observed that the bioassay conditions described in the Ph. Eur. 6. are not suitable for the measurement of concentration change experienced during a stability test. The aim of our study was to optimize the chemical and microbiological methods. We have improved the eluent system based on earlier HPLC methods for the separation of the main heptaene and the minor tetraene by-constituents in Fungizone (Bristol-Myers Squibb). The most optimal bioassay conditions were determined where a relatively wide concentration range can be measured. With the improved methods both chemical and microbiological changes can be more accurately measured in our future stability tests.",
author = "Fittler Andr{\'a}s and Z. Matus and Kocsis B{\'e}la and Botz Lajos",
year = "2008",
language = "Hungarian",
volume = "78",
pages = "95--102",
journal = "Acta Pharmaceutica Hungarica",
issn = "0001-6659",
publisher = "Magyar Gyogyszereszeti Tarsasag",
number = "3",

}

TY - JOUR

T1 - Amfotericin B tartalmú készítmények kvantitatív analízisének kémiai és mikrobiológiai vonatkozásai

AU - András, Fittler

AU - Matus, Z.

AU - Béla, Kocsis

AU - Lajos, Botz

PY - 2008

Y1 - 2008

N2 - Amphotericin B can be determined by chemical (HPLC, spectrophotometry) and microbiological (bioassay) methods. The utilization of both during a stability test can give more detailed information about the activity and concentration change of amphotericin B solutions. Previously published HPLC methods do not lay stress on the separation of by-constituents present in the substance. We have also observed that the bioassay conditions described in the Ph. Eur. 6. are not suitable for the measurement of concentration change experienced during a stability test. The aim of our study was to optimize the chemical and microbiological methods. We have improved the eluent system based on earlier HPLC methods for the separation of the main heptaene and the minor tetraene by-constituents in Fungizone (Bristol-Myers Squibb). The most optimal bioassay conditions were determined where a relatively wide concentration range can be measured. With the improved methods both chemical and microbiological changes can be more accurately measured in our future stability tests.

AB - Amphotericin B can be determined by chemical (HPLC, spectrophotometry) and microbiological (bioassay) methods. The utilization of both during a stability test can give more detailed information about the activity and concentration change of amphotericin B solutions. Previously published HPLC methods do not lay stress on the separation of by-constituents present in the substance. We have also observed that the bioassay conditions described in the Ph. Eur. 6. are not suitable for the measurement of concentration change experienced during a stability test. The aim of our study was to optimize the chemical and microbiological methods. We have improved the eluent system based on earlier HPLC methods for the separation of the main heptaene and the minor tetraene by-constituents in Fungizone (Bristol-Myers Squibb). The most optimal bioassay conditions were determined where a relatively wide concentration range can be measured. With the improved methods both chemical and microbiological changes can be more accurately measured in our future stability tests.

UR - http://www.scopus.com/inward/record.url?scp=55349145643&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=55349145643&partnerID=8YFLogxK

M3 - Article

VL - 78

SP - 95

EP - 102

JO - Acta Pharmaceutica Hungarica

JF - Acta Pharmaceutica Hungarica

SN - 0001-6659

IS - 3

ER -