Charge-transfer chromatographic study of the interaction of antibiotics with sodium dodecylsulfate

Esther Forgács, Tibor Csethati

Research output: Contribution to journalArticle

2 Citations (Scopus)


The interaction of 29 antibiotics with the anionic surfactant sodium dodecylsulfate (SDS) was studied by charge-transfer reversed-phase chromatography carried out on impregnated silica layers using water-methanol mixtures as eluents. The hydrophobicity of antibiotics and the relative strength of SDS-antibiotic interaction was calculated separately for each antibiotic-SDS pair. SDS interacted with 17 antibiotics where the antibiotic-SDS complex was either more hydrophillic or more hydrophobic than the uncomplexed molecule. The relative strength of interaction depended considerably on the molecular structure of the antibiotics. No significant linear correlation was found between the hydrophobicity parameters of antibiotics and their capacity to interact with SDS. Stepwise regression analysis proved that the inductive effect of substituents, their electron-withdrawing power and proton-acceptor capacity exert a significance influence on the strength of interaction.

Original languageEnglish
Pages (from-to)1295-1302
Number of pages8
JournalJournal of Pharmaceutical and Biomedical Analysis
Issue number9-10
Publication statusPublished - Jun 1 1997


  • Antibiotics
  • Charge-transfer chromatography
  • Sodium dodecylsulfate

ASJC Scopus subject areas

  • Analytical Chemistry
  • Pharmaceutical Science
  • Drug Discovery
  • Spectroscopy
  • Clinical Biochemistry

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