Charge transfer chromatographic study of the binding of commercial pesticides to various albumins

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

The interaction of 28 commercial pesticides with human and bovine serum albumin as well as with egg albumin was studied by charge-transfer reversed-phase thin-layer chromatography and the relative strength of the interaction was calculated. Only one pesticide interacted with egg albumin whereas the majority of pesticides bound both to bovine and human serum albumins. Stepwise regression analysis proved that the hydrophobicity parameters of pesticides exert a significant impact on their capacity to bind to serum albumins. These findings support the hypothesis that the binding of pesticides to albumins may involve hydrophilic forces occurring between the corresponding apolar substructures of pesticides and amino acid side chains. No linear correlation was found between the capacities of human and bovine serum albumins to bind pesticides.

Original languageEnglish
Pages (from-to)285-290
Number of pages6
JournalJournal of Chromatography A
Volume699
Issue number1-2
DOIs
Publication statusPublished - May 5 1995

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Pesticides
Charge transfer
Albumins
Bovine Serum Albumin
Serum Albumin
Ovum
Thin layer chromatography
Reverse-Phase Chromatography
Hydrophobicity
Thin Layer Chromatography
Hydrophobic and Hydrophilic Interactions
Regression analysis
Regression Analysis
Amino Acids

ASJC Scopus subject areas

  • Biochemistry
  • Analytical Chemistry
  • Organic Chemistry

Cite this

Charge transfer chromatographic study of the binding of commercial pesticides to various albumins. / Cserháti, T.; Forgács, E.

In: Journal of Chromatography A, Vol. 699, No. 1-2, 05.05.1995, p. 285-290.

Research output: Contribution to journalArticle

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