Charge motions studied in the bacteriorhodopsin mutants D85N and D212N

Csilla Gergely, Constanta Ganea, Sándor Száraz, G. Váró

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

The photoelectric response signals of the D85N and D212N bacteriorhodopsin mutants expressed in a Halobacterium halobium strain were measured. The purple membranes containing only the mutant protein were oriented and immobilized in polycrylamide gel. The absorption changes and electric response signals were measured after laser excitation, enabling the related charge motions in the protein to be followed during the photocycle. The signals were analysed on the basis of the sequential model with reversible reactions for the photocycle of the mutants. The transition lifetimes and electrogenicity of the intermediates were determined. From this analysis the relative electrogenicity of the L intermediate was established. The results corroborate the existence of a conformational change during the photocycle in these bacteriorhodopsin mutants.

Original languageEnglish
Pages (from-to)27-32
Number of pages6
JournalJournal of Photochemistry and Photobiology, B: Biology
Volume27
Issue number1
DOIs
Publication statusPublished - 1995

Fingerprint

Bacteriorhodopsins
Purple Membrane
Halobacterium salinarum
Proteins
mutants
Laser excitation
Mutant Proteins
proteins
Halobacterium halobium
Lasers
Gels
Membranes
gels
membranes
lasers
life (durability)
excitation
bacteriorhodopsins

Keywords

  • Halobacteria
  • Photocurrent
  • Photocycle
  • Retinal protein

ASJC Scopus subject areas

  • Biophysics
  • Radiology Nuclear Medicine and imaging
  • Radiation
  • Radiological and Ultrasound Technology
  • Plant Science
  • Bioengineering
  • Physical and Theoretical Chemistry

Cite this

Charge motions studied in the bacteriorhodopsin mutants D85N and D212N. / Gergely, Csilla; Ganea, Constanta; Száraz, Sándor; Váró, G.

In: Journal of Photochemistry and Photobiology, B: Biology, Vol. 27, No. 1, 1995, p. 27-32.

Research output: Contribution to journalArticle

Gergely, Csilla ; Ganea, Constanta ; Száraz, Sándor ; Váró, G. / Charge motions studied in the bacteriorhodopsin mutants D85N and D212N. In: Journal of Photochemistry and Photobiology, B: Biology. 1995 ; Vol. 27, No. 1. pp. 27-32.
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