The photocycle and the corresponding electric signals from vacuum-dried bacteriorhodopsin were studied. For this purpose, oriented purple membrane samples were used. The absorption changes resulting from laser flash excitation and electric response signals were measured with a time resolution of 0.1 μs. Two intermediates absorbing at 630 and 412 nm were seen. The decay of the 630 nm form cannot be described by a single exponential. From the electric signals it can be calculated that the proton does not leave the membrane, and only undergoes a backward and forward motion with respect to the proton-pumping direction of the bacteriorhodopsin.
|Number of pages||6|
|Journal||Journal of Photochemistry and Photobiology, B: Biology|
|Publication status||Published - May 1988|
- Schiff base
- role of water.
ASJC Scopus subject areas
- Radiological and Ultrasound Technology
- Radiology Nuclear Medicine and imaging