Charge motion in vacuum-dried bacteriorhodopsin

I. Kovács, G. Váró

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

The photocycle and the corresponding electric signals from vacuum-dried bacteriorhodopsin were studied. For this purpose, oriented purple membrane samples were used. The absorption changes resulting from laser flash excitation and electric response signals were measured with a time resolution of 0.1 μs. Two intermediates absorbing at 630 and 412 nm were seen. The decay of the 630 nm form cannot be described by a single exponential. From the electric signals it can be calculated that the proton does not leave the membrane, and only undergoes a backward and forward motion with respect to the proton-pumping direction of the bacteriorhodopsin.

Original languageEnglish
Pages (from-to)469-474
Number of pages6
JournalJournal of Photochemistry and Photobiology, B: Biology
Volume1
Issue number4
DOIs
Publication statusPublished - May 1988

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Keywords

  • Bacteriorhodopsin
  • Schiff base
  • photocycle
  • role of water.

ASJC Scopus subject areas

  • Radiation
  • Radiological and Ultrasound Technology
  • Biophysics
  • Radiology Nuclear Medicine and imaging

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