Characterization of yeast isolates originating from Hungarian dairy products using traditional and molecular identification techniques

R. Vasdinyei, T. Deák

Research output: Contribution to journalArticle

67 Citations (Scopus)

Abstract

Yeast isolates collected from various Hungarian dairy products were identified using simplified identification system (SIM) and restriction fragment analysis of PCR-amplified 18S rDNA with the neighbouring ITS1 region (ITS-PCR; ribotyping). The isolates were grouped into 26 species; Geotrichum candidum, Debaryomyces hansenii, Yarrowia lipolytica, Kluyveromyces lactis and Candida catenulata were found as the predominant species. SIM and ITS-PCR proved to be useful and convenient taxonomic tools for rapid identification at species level. Two most frequent species, G. candidum and D. hansenii, were further characterized by randomly amplified polymorphic DNA (RAPD-PCR) analysis. RAPD-PCR using M13 primer resulted in discrimination between most strains of the same species and allowed a certain degree of intraspecific typing.

Original languageEnglish
Pages (from-to)123-130
Number of pages8
JournalInternational Journal of Food Microbiology
Volume86
Issue number1-2
DOIs
Publication statusPublished - Sep 1 2003

Fingerprint

Geotrichum candidum
Debaryomyces hansenii
Dairy Products
Random Amplified Polymorphic DNA Technique
dairy products
Yeasts
yeasts
Yarrowia lipolytica
Kluyveromyces marxianus var. lactis
Polymerase Chain Reaction
Candida
DNA fingerprinting
Yarrowia
Geotrichum
Kluyveromyces
Ribotyping
Ribosomal DNA
DNA
methodology

Keywords

  • Dairy yeasts
  • Identification
  • ITS-PCR
  • RAPD

ASJC Scopus subject areas

  • Food Science
  • Microbiology

Cite this

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