Characterization of the selenocysteine-containing metabolome in selenium-rich yeast: Part 1. Identification of new species by multi-dimensional liquid chromatography with parallel ICP-MS and electrospray Q-TOFMS/MS detection

M. Dernovics, Ryszard Lobinski

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25 Citations (Scopus)

Abstract

Size-exclusion chromatography (SEC)-strong anion-exchange (SAX) HPLC fractionation of selenium species from the aqueous extract of a selenised yeast sample was optimised under the strict selenium mass balance control by ICP-MS. The SAX HPLC-ICP-MS chromatogram of the most intense SEC fraction produced seven peaks. They were all successfully identified by reversed phase (RP) nanoHPLC-electrospray Q-TOFMS/MS. Eight Se-compounds (derivatives of glutathione) were identified: six of them have not been reported previously. Six of the identified compounds contained selenocysteine (28% of the water-soluble selenium), stabilised by either Se-S or Se-Se bridges. The extensive MS/MS data presented are potentially useful for the optimization of direct LC-ESIMS/MS analyses in the selected (SRM) or multiple (MRM) reaction monitoring modes for the purpose of the authenticity and quality control of Se-rich yeast supplements.

Original languageEnglish
Pages (from-to)72-83
Number of pages12
JournalJournal of Analytical Atomic Spectrometry
Volume23
Issue number1
DOIs
Publication statusPublished - 2008

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Selenocysteine
Liquid chromatography
Selenium
Yeast
Size exclusion chromatography
Anions
Fractionation
Quality control
Glutathione
Ion exchange
Derivatives
Water
Monitoring

ASJC Scopus subject areas

  • Spectroscopy

Cite this

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title = "Characterization of the selenocysteine-containing metabolome in selenium-rich yeast: Part 1. Identification of new species by multi-dimensional liquid chromatography with parallel ICP-MS and electrospray Q-TOFMS/MS detection",
abstract = "Size-exclusion chromatography (SEC)-strong anion-exchange (SAX) HPLC fractionation of selenium species from the aqueous extract of a selenised yeast sample was optimised under the strict selenium mass balance control by ICP-MS. The SAX HPLC-ICP-MS chromatogram of the most intense SEC fraction produced seven peaks. They were all successfully identified by reversed phase (RP) nanoHPLC-electrospray Q-TOFMS/MS. Eight Se-compounds (derivatives of glutathione) were identified: six of them have not been reported previously. Six of the identified compounds contained selenocysteine (28{\%} of the water-soluble selenium), stabilised by either Se-S or Se-Se bridges. The extensive MS/MS data presented are potentially useful for the optimization of direct LC-ESIMS/MS analyses in the selected (SRM) or multiple (MRM) reaction monitoring modes for the purpose of the authenticity and quality control of Se-rich yeast supplements.",
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AU - Lobinski, Ryszard

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