Characterization of the selectivity of a phenytoin imprinted polymer

Tímea Pap, George Horvai

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

The selectivity of analytical methods based on molecularly imprinted polymers (MIPs) is due to the preferential adsorption of the analyte(s) as compared to other substances (interferences). This paper shows the theoretical and practical difficulties, which have to be considered and solved when real samples need to be analysed in a wide range of analyte and interferant concentrations. It is shown that the estimation of interference effects requires either many measurements or a realistic model of the adsorption equilibrium in mixed solutions of the analyte and the interferences. Examples are shown for positive (cooperative) interference effects, for better experimental design and interpretation of binary isotherm measurements and for establishing the chemical model of interference from selectivity measurements. The usual MIP model consisting of a cavity, which closely fits the shape of the template from all sides, appears unsuitable for this MIP, and it is replaced with a more realistic, more open model. The applicability of the results to using non-imprinted polymers as selective sorbents and to screening drug candidates is also shown.

Original languageEnglish
Pages (from-to)99-107
Number of pages9
JournalJournal of Chromatography A
Volume1034
Issue number1-2
DOIs
Publication statusPublished - Apr 23 2004

Keywords

  • Adsorption
  • Binding studies
  • Molecularly imprinting
  • Phenytoin
  • Selectivity
  • Stationary phases, LC

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Organic Chemistry

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