Characterization of the mouse gene that encodes the δ/YY1/NF-E1/UCRBP transcription factor

Géza Sáfrány, Robert P. Perry

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

The mouse gene that encodes the δ transcription factor has been cloned and characterized. This gene spans 23 kb and is composed of five exons and four introns. The first exon consists of a long (431 bp), (C+G)-rich, untranslated segment and a 679-bp coding segment, which specifies the unusual tracts of consecutive acidic residues and histidines and the long alanine-glycine stretches. The sequence that encodes the four zinc-finger motifs of this protein is interrupted by two introns. Nuclease protection experiments revealed a major transcriptional start point and several additional start points distributed over a 28-bp segment. Transfection experiments with 5′ and 3′ deletion mutants localized the promoter to a (C+G)-rich region that is <700 bp upstream and no more than 32 bp downstream of the major start point. An especially critical promoter element lies between -58 and -18 and contains a high-affinity Sp1 binding site, as demonstrated by electrophoretic mobility-shift experiments with nuclear extract and recombinant Sp1 proteins. Several striking similarities between the δ gene and genes encoding other transcription factors and regulatory proteins are noted and discussed with respect to their possible biological significance.

Original languageEnglish
Pages (from-to)5559-5563
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume90
Issue number12
DOIs
Publication statusPublished - Jun 15 1993

Keywords

  • (G+C)-rich promoter
  • CpG suppression
  • Sp1 site
  • Zinc finger protein

ASJC Scopus subject areas

  • General

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