Characterization of the gene for factor C, an extracellular signal protein involved in morphological differentiation of Streptomyces griseus

Zsuzsa Birkó, Andrea Sümegi, Andrea Vinnai, Gilles Van Wezel, Ferenc Szeszák, Sándor Vitális, P. Szabó, Z. Kele, T. Janáky, Sándor Biró

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

The gene encoding factor C (facC), an extracellular signal protein involved in cellular differentiation, was cloned from Streptomyces griseus 45H, and the complete nucleotide sequence was determined. The deduced amino acid sequence was confirmed by HPLC/electrospray ionization-mass spectrometry analysis. The full-length protein consists of 324 amino acids and has a predicted molecular mass of 34,523 Da. The mature extracellular 286 amino acid protein (31,038 Da) is probably produced by cleaving off a 38 amino acid secretion signal sequence. Southern hybridization detected facC in several other Streptomyces strains, but database searches failed to identify a protein with significant homology to factor C. Expression of facC from a low-copy-number vector in S. griseus 52-1 resulted in a phenotypic effect similar to that given by exogenously added factor C protein.

Original languageEnglish
Pages (from-to)2245-2253
Number of pages9
JournalMicrobiology
Volume145
Issue number9
Publication statusPublished - Sep 1999

Fingerprint

Streptomyces griseus
Amino Acids
Genes
Proteins
Electrospray Ionization Mass Spectrometry
Streptomyces
Protein Sorting Signals
Protein C
Amino Acid Sequence
High Pressure Liquid Chromatography
Databases

Keywords

  • Autoregulator
  • Cell differentiation
  • Extracellular signalling
  • Sporulation
  • Streptomyces

ASJC Scopus subject areas

  • Microbiology

Cite this

Birkó, Z., Sümegi, A., Vinnai, A., Van Wezel, G., Szeszák, F., Vitális, S., ... Biró, S. (1999). Characterization of the gene for factor C, an extracellular signal protein involved in morphological differentiation of Streptomyces griseus. Microbiology, 145(9), 2245-2253.

Characterization of the gene for factor C, an extracellular signal protein involved in morphological differentiation of Streptomyces griseus. / Birkó, Zsuzsa; Sümegi, Andrea; Vinnai, Andrea; Van Wezel, Gilles; Szeszák, Ferenc; Vitális, Sándor; Szabó, P.; Kele, Z.; Janáky, T.; Biró, Sándor.

In: Microbiology, Vol. 145, No. 9, 09.1999, p. 2245-2253.

Research output: Contribution to journalArticle

Birkó, Z, Sümegi, A, Vinnai, A, Van Wezel, G, Szeszák, F, Vitális, S, Szabó, P, Kele, Z, Janáky, T & Biró, S 1999, 'Characterization of the gene for factor C, an extracellular signal protein involved in morphological differentiation of Streptomyces griseus', Microbiology, vol. 145, no. 9, pp. 2245-2253.
Birkó Z, Sümegi A, Vinnai A, Van Wezel G, Szeszák F, Vitális S et al. Characterization of the gene for factor C, an extracellular signal protein involved in morphological differentiation of Streptomyces griseus. Microbiology. 1999 Sep;145(9):2245-2253.
Birkó, Zsuzsa ; Sümegi, Andrea ; Vinnai, Andrea ; Van Wezel, Gilles ; Szeszák, Ferenc ; Vitális, Sándor ; Szabó, P. ; Kele, Z. ; Janáky, T. ; Biró, Sándor. / Characterization of the gene for factor C, an extracellular signal protein involved in morphological differentiation of Streptomyces griseus. In: Microbiology. 1999 ; Vol. 145, No. 9. pp. 2245-2253.
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