Characterization of the complex interaction between the electron acceptor silicomolybdate and Photosystem II

G. Schansker, Jack J S van Rensen

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Silicomolybdate (SiMo) and its effects on thylakoids have been characterized to evaluate its use as a probe for Photosystem II (PS II). It can accept electrons at two places in the electron transport chain: one at PS II and the other at PS I. In the presence of 1 μM 2,5-dibromo-3-methyl-6-isopropyl-p-benzoquinone (DBMIB) only the site at PS II is available. It is suggested that SiMo must disp;ace bicarbonate from its binding site to be able to function as an electron acceptor. This displacement is non-competitive. The binding of SiMo is inhibited differentially by PS II inhibitors: dinoseb>ioxynil> diuron. This difference is determined by the different positions of the inhibitors within the QB binding niche and their interaction with bicarbonate. The experimental results show that the SiMo-binding niche is located between the parallel helices of the D1 and D2 proteins of PS II, close to the non-heme iron. We conclude that SiMo is an electron acceptor with unique characteristics useful as a probe of the acceptor side of PS II.

Original languageEnglish
Pages (from-to)165-175
Number of pages11
JournalPhotosynthesis Research
Volume37
Issue number2
DOIs
Publication statusPublished - Aug 1993

Fingerprint

Photosystem II Protein Complex
photosystem II
electrons
Electrons
Bicarbonates
bicarbonates
Dibromothymoquinone
niches
dinoseb
ioxynil
D2 protein
Diuron
benzoquinones
diuron
D1 protein
Thylakoids
electron transport chain
Electron Transport
thylakoids
silicomolybdate

Keywords

  • 2,5-dibromo-3-methyl-6-isopropyl-p-benzoquinone
  • bicarbonate effect
  • diuron, non-heme iron
  • triazine resistance

ASJC Scopus subject areas

  • Plant Science

Cite this

Characterization of the complex interaction between the electron acceptor silicomolybdate and Photosystem II. / Schansker, G.; van Rensen, Jack J S.

In: Photosynthesis Research, Vol. 37, No. 2, 08.1993, p. 165-175.

Research output: Contribution to journalArticle

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