Characterization of the APC10/DOC1 subunit of the anaphase promoting complex in Drosophila melanogaster

Margit Pál, Kata Varga, Olga Nagy, P. Deák

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

The anaphase promoting complex or cyclosome (APC/C) is a large protein complex with an ubiquitin ligase activity which specifically targets mitotic regulatory proteins for proteasomal degradation. The APC/C contains at least 11 subunits, most of which are evolutionarily conserved from yeasts to humans. We have isolated and characterized mutant alleles of the gene that codes for the APC10/Doc1 subunit of the Drosophila APC/C. Loss of function APC10/Doc1 mutants have rudimentary imaginal discs and arrest their development as prepupae. Larval neuroblasts from these mutants show gross mitotic defects including high mitotic index, chromosome overcondensation, metaphase-like arrest and frequent aneuploid and polyploid cells. Mitotically arrested cells accumulate one of the main substrates of APC/C, cyclin B, most likely due to disabled ubiquitination activity. Our results suggest that the Apc10/Doc1 subunit has an essential role in establishing E3 ubiquitin ligase activity of APC/C in Drosophila.

Original languageEnglish
Pages (from-to)51-64
Number of pages14
JournalActa Biologica Hungarica
Volume58
Issue numberSUPPL. 1
DOIs
Publication statusPublished - Nov 2007

Fingerprint

Anaphase-Promoting Complex-Cyclosome
Drosophila melanogaster
Ubiquitin-Protein Ligase Complexes
mutants
Drosophila
protein
Imaginal Discs
Cyclin B
imaginal discs
prepupae
ubiquitin-protein ligase
Mitotic Index
Polyploidy
Ubiquitin-Protein Ligases
yeast
defect
chromosome
Ubiquitination
regulatory proteins
aneuploidy

Keywords

  • APC/C
  • Apc10/Doc1
  • Homologous recombination

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)

Cite this

Characterization of the APC10/DOC1 subunit of the anaphase promoting complex in Drosophila melanogaster. / Pál, Margit; Varga, Kata; Nagy, Olga; Deák, P.

In: Acta Biologica Hungarica, Vol. 58, No. SUPPL. 1, 11.2007, p. 51-64.

Research output: Contribution to journalArticle

@article{a088830b646340e3af8d2a927b2cbd78,
title = "Characterization of the APC10/DOC1 subunit of the anaphase promoting complex in Drosophila melanogaster",
abstract = "The anaphase promoting complex or cyclosome (APC/C) is a large protein complex with an ubiquitin ligase activity which specifically targets mitotic regulatory proteins for proteasomal degradation. The APC/C contains at least 11 subunits, most of which are evolutionarily conserved from yeasts to humans. We have isolated and characterized mutant alleles of the gene that codes for the APC10/Doc1 subunit of the Drosophila APC/C. Loss of function APC10/Doc1 mutants have rudimentary imaginal discs and arrest their development as prepupae. Larval neuroblasts from these mutants show gross mitotic defects including high mitotic index, chromosome overcondensation, metaphase-like arrest and frequent aneuploid and polyploid cells. Mitotically arrested cells accumulate one of the main substrates of APC/C, cyclin B, most likely due to disabled ubiquitination activity. Our results suggest that the Apc10/Doc1 subunit has an essential role in establishing E3 ubiquitin ligase activity of APC/C in Drosophila.",
keywords = "APC/C, Apc10/Doc1, Homologous recombination",
author = "Margit P{\'a}l and Kata Varga and Olga Nagy and P. De{\'a}k",
year = "2007",
month = "11",
doi = "10.1556/ABiol.58.2007.Suppl.5",
language = "English",
volume = "58",
pages = "51--64",
journal = "Acta Biologica Hungarica",
issn = "0236-5383",
publisher = "Akademiai Kiado",
number = "SUPPL. 1",

}

TY - JOUR

T1 - Characterization of the APC10/DOC1 subunit of the anaphase promoting complex in Drosophila melanogaster

AU - Pál, Margit

AU - Varga, Kata

AU - Nagy, Olga

AU - Deák, P.

PY - 2007/11

Y1 - 2007/11

N2 - The anaphase promoting complex or cyclosome (APC/C) is a large protein complex with an ubiquitin ligase activity which specifically targets mitotic regulatory proteins for proteasomal degradation. The APC/C contains at least 11 subunits, most of which are evolutionarily conserved from yeasts to humans. We have isolated and characterized mutant alleles of the gene that codes for the APC10/Doc1 subunit of the Drosophila APC/C. Loss of function APC10/Doc1 mutants have rudimentary imaginal discs and arrest their development as prepupae. Larval neuroblasts from these mutants show gross mitotic defects including high mitotic index, chromosome overcondensation, metaphase-like arrest and frequent aneuploid and polyploid cells. Mitotically arrested cells accumulate one of the main substrates of APC/C, cyclin B, most likely due to disabled ubiquitination activity. Our results suggest that the Apc10/Doc1 subunit has an essential role in establishing E3 ubiquitin ligase activity of APC/C in Drosophila.

AB - The anaphase promoting complex or cyclosome (APC/C) is a large protein complex with an ubiquitin ligase activity which specifically targets mitotic regulatory proteins for proteasomal degradation. The APC/C contains at least 11 subunits, most of which are evolutionarily conserved from yeasts to humans. We have isolated and characterized mutant alleles of the gene that codes for the APC10/Doc1 subunit of the Drosophila APC/C. Loss of function APC10/Doc1 mutants have rudimentary imaginal discs and arrest their development as prepupae. Larval neuroblasts from these mutants show gross mitotic defects including high mitotic index, chromosome overcondensation, metaphase-like arrest and frequent aneuploid and polyploid cells. Mitotically arrested cells accumulate one of the main substrates of APC/C, cyclin B, most likely due to disabled ubiquitination activity. Our results suggest that the Apc10/Doc1 subunit has an essential role in establishing E3 ubiquitin ligase activity of APC/C in Drosophila.

KW - APC/C

KW - Apc10/Doc1

KW - Homologous recombination

UR - http://www.scopus.com/inward/record.url?scp=38749140994&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=38749140994&partnerID=8YFLogxK

U2 - 10.1556/ABiol.58.2007.Suppl.5

DO - 10.1556/ABiol.58.2007.Suppl.5

M3 - Article

C2 - 18297794

AN - SCOPUS:38749140994

VL - 58

SP - 51

EP - 64

JO - Acta Biologica Hungarica

JF - Acta Biologica Hungarica

SN - 0236-5383

IS - SUPPL. 1

ER -