Characterization of the aodA, dnmA, mnSOD and pimA genes in Aspergillus nidulans

E. Leiter, Hee Soo Park, Nak Jung Kwon, Kap Hoon Han, T. Emri, Viktor Olah, I. Mészáros, Beatrix Dienes, Janos Vincze, L. Csernoch, Jae Hyuk Yu, I. Pócsi

Research output: Contribution to journalArticle

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Abstract

Mitochondria play key roles in cellular energy generation and lifespan of most eukaryotes. To understand the functions of four nuclear-encoded genes predicted to be related to the maintenance of mitochondrial morphology and function in Aspergillus nidulans, systematic characterization was carried out. The deletion and overexpression mutants of aodA, dnmA, mnSOD and pimA encoding alternative oxidase, dynamin related protein, manganese superoxide dismutase and Lon protease, respectively, were generated and examined for their growth, stress tolerances, respiration, autolysis, cell death, sterigmatocystin production, hyphal morphology and size, and mitochondrial superoxide production as well as development. Overall, genetic manipulation of these genes had less effect on cellular physiology and ageing in A. nidulans than that of their homologs in another fungus Podospora anserina with a well-characterized senescence. The observed interspecial phenotypic differences can be explained by the dissimilar intrinsic stabilities of the mitochondrial genomes in A. nidulans and P. anserina. Furthermore, the marginally altered phenotypes observed in A. nidulans mutants indicate the presence of effective compensatory mechanisms for the complex networks of mitochondrial defense and quality control. Importantly, these findings can be useful for developing novel platforms for heterologous protein production, or on new biocontrol and bioremediation technologies based on Aspergillus species.

Original languageEnglish
Article number20523
JournalScientific Reports
Volume6
DOIs
Publication statusPublished - Feb 5 2016

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Potassium Iodide
Aspergillus nidulans
Podospora
Genes
Protease La
Sterigmatocystin
Mitochondrial Size
Dynamins
Autolysis
Environmental Biodegradation
Mitochondrial Genome
Cell Aging
Aspergillus
Eukaryota
Superoxides
Quality Control
Superoxide Dismutase
Mitochondria
Respiration
Proteins

ASJC Scopus subject areas

  • General

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Characterization of the aodA, dnmA, mnSOD and pimA genes in Aspergillus nidulans. / Leiter, E.; Park, Hee Soo; Kwon, Nak Jung; Han, Kap Hoon; Emri, T.; Olah, Viktor; Mészáros, I.; Dienes, Beatrix; Vincze, Janos; Csernoch, L.; Yu, Jae Hyuk; Pócsi, I.

In: Scientific Reports, Vol. 6, 20523, 05.02.2016.

Research output: Contribution to journalArticle

Leiter, E. ; Park, Hee Soo ; Kwon, Nak Jung ; Han, Kap Hoon ; Emri, T. ; Olah, Viktor ; Mészáros, I. ; Dienes, Beatrix ; Vincze, Janos ; Csernoch, L. ; Yu, Jae Hyuk ; Pócsi, I. / Characterization of the aodA, dnmA, mnSOD and pimA genes in Aspergillus nidulans. In: Scientific Reports. 2016 ; Vol. 6.
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