Characterization of the 820-nm transmission signal paralleling the chlorophyll a fluorescence rise (OJIP) in pea leaves

Gert Schansker, Alaka Srivastava, Govindjee, Reto J. Strasser

Research output: Contribution to journalArticle

204 Citations (Scopus)


Monitoring transmission changes at 820 nm, a measure of the redox states of plastocyanin (PC) and P700, is a good complementary technique for chlorophyll (chl) a fluorescence induction measurements. A thorough characterization of the properties of the 820-nm transmission kinetics during the first second after a dark-to-light transition is provided here for pea (Pisum sativum L.) leaves. The data indicate that plastocyanin in a dark-adapted leaf is in the reduced state. Three photosystem I (PSI)-related components, PC, P700 and ferredoxin, can contribute to the 820-nm transmission signal. The contribution of ferredoxin, however, is only approximately 5%, thus, it can be neglected for further analysis. Here, we show that by monitoring the sequential oxidation of PC and P700 during a far-red pulse and analysing the re-reduction kinetics it is possible to assign the three re-reduction components to PC (τ = 7-14 s) and P700 (τ = 35-55 ms and 1.2-1.6 s). Our data indicate that the faster re-reduction phase (τ = 35-55 ms) may represent a recombination reaction between P700+ and the acceptor side of PSI. This information made it possible to show that the ratio between the potential contributions of PC:P700 is 50:50 in pea and Camellia leaves and 40:60 in sugar beet leaves.

Original languageEnglish
Pages (from-to)785-796
Number of pages12
JournalFunctional Plant Biology
Issue number7
Publication statusPublished - Sep 11 2003


  • 820-nm transmission
  • Chlorophyll fluorescence
  • DCMU
  • P700
  • Pea
  • Plastocyanin
  • Plastoquinone pool
  • Re-reduction kinetics
  • Stromal electrons

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Plant Science

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