Characterization of stress responses of heavy metal and metalloid inducible promoters in synechocystis PCC6803

Barbara Blasi, Loredana Peca, Imre Vass, Peter B. Kós

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

In several biotechnological applications of living bacterial cells with inducible gene expression systems, the extent of overexpression and the specificity to the inducer are key elements. In the present study, we established the concentration ranges of Zn 2+, Ni 2+, Co 2+, AsO 2 -, and Cd 2+ ions that caused significant activation of the respective promoters of Synechocystis sp. without concomitant unspecific stress responses. The low expression levels can be increased up to 10-100-fold upon treatments with Cd 2+, AsO 2 -, Zn 2+, and Co 2+ ions and up to 800-fold upon Ni 2+ treatment. These results facilitate the development of conditional gene expression systems in cyanobacteria.

Original languageEnglish
Pages (from-to)166-169
Number of pages4
JournalJournal of Microbiology and Biotechnology
Volume22
Issue number2
DOIs
Publication statusPublished - Feb 1 2012

    Fingerprint

Keywords

  • Cyanobacterial gene expression
  • Heavy metal toxicity
  • Inducible promoters
  • Specificity of promoters
  • Synechocystis PCC6803

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology

Cite this