Characterization of stress processes of Phaffia rhodozyma stress-resistant mutant

E. Horváth, G. Papp, Z. Gazdag, J. Belágyi, Á Blaskó, J. Deli, C. Vágvölgyi, M. Pesti

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

A carotenoid-less Phaffia rhodozyma mutant (MCP 325) exhibited significantly higher resistance to oxidative stressors such as menadione, H 2O2 and K2Cr2O7 than its astaxanthin-producing parental strain (MCP 324). The absence of carotenoids in the mutant did not explain this phenomenon. The cause of the decreased superoxide, hydroxyl radical and glutathione contents, the increased peroxide concentration and the elevated specific activity of catalase under uninduced conditions may be a second mutation. Peroxide treatment induced specific catalase activity in the mutant but not in the parental strain. Regulation of these processes led to the result that, in spite of the mutations, the two strains exhibited the same multiplication rate and generation time.

Original languageEnglish
Pages (from-to)204-210
Number of pages7
JournalActa Biologica Hungarica
Volume62
Issue number2
DOIs
Publication statusPublished - Jun 1 2011

Fingerprint

Peroxides
Carotenoids
Catalase
Potassium Dichromate
carotenoid
Vitamin K 3
Mutation
mutation
Superoxides
Hydroxyl Radical
Glutathione
generation time
hydroxyl radical
mutant
astaxanthine

Keywords

  • carotenoid
  • EPR
  • GSH
  • oxidative stress
  • Phaffia rhodozyma

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Environmental Science(all)
  • Neurology

Cite this

Characterization of stress processes of Phaffia rhodozyma stress-resistant mutant. / Horváth, E.; Papp, G.; Gazdag, Z.; Belágyi, J.; Blaskó, Á; Deli, J.; Vágvölgyi, C.; Pesti, M.

In: Acta Biologica Hungarica, Vol. 62, No. 2, 01.06.2011, p. 204-210.

Research output: Contribution to journalArticle

@article{c699bfc2f37041afba19ac3c7ff77285,
title = "Characterization of stress processes of Phaffia rhodozyma stress-resistant mutant",
abstract = "A carotenoid-less Phaffia rhodozyma mutant (MCP 325) exhibited significantly higher resistance to oxidative stressors such as menadione, H 2O2 and K2Cr2O7 than its astaxanthin-producing parental strain (MCP 324). The absence of carotenoids in the mutant did not explain this phenomenon. The cause of the decreased superoxide, hydroxyl radical and glutathione contents, the increased peroxide concentration and the elevated specific activity of catalase under uninduced conditions may be a second mutation. Peroxide treatment induced specific catalase activity in the mutant but not in the parental strain. Regulation of these processes led to the result that, in spite of the mutations, the two strains exhibited the same multiplication rate and generation time.",
keywords = "carotenoid, EPR, GSH, oxidative stress, Phaffia rhodozyma",
author = "E. Horv{\'a}th and G. Papp and Z. Gazdag and J. Bel{\'a}gyi and {\'A} Blask{\'o} and J. Deli and C. V{\'a}gv{\"o}lgyi and M. Pesti",
year = "2011",
month = "6",
day = "1",
doi = "10.1556/ABiol.62.2011.2.10",
language = "English",
volume = "62",
pages = "204--210",
journal = "Acta Biologica Hungarica",
issn = "0236-5383",
publisher = "Akademiai Kiado",
number = "2",

}

TY - JOUR

T1 - Characterization of stress processes of Phaffia rhodozyma stress-resistant mutant

AU - Horváth, E.

AU - Papp, G.

AU - Gazdag, Z.

AU - Belágyi, J.

AU - Blaskó, Á

AU - Deli, J.

AU - Vágvölgyi, C.

AU - Pesti, M.

PY - 2011/6/1

Y1 - 2011/6/1

N2 - A carotenoid-less Phaffia rhodozyma mutant (MCP 325) exhibited significantly higher resistance to oxidative stressors such as menadione, H 2O2 and K2Cr2O7 than its astaxanthin-producing parental strain (MCP 324). The absence of carotenoids in the mutant did not explain this phenomenon. The cause of the decreased superoxide, hydroxyl radical and glutathione contents, the increased peroxide concentration and the elevated specific activity of catalase under uninduced conditions may be a second mutation. Peroxide treatment induced specific catalase activity in the mutant but not in the parental strain. Regulation of these processes led to the result that, in spite of the mutations, the two strains exhibited the same multiplication rate and generation time.

AB - A carotenoid-less Phaffia rhodozyma mutant (MCP 325) exhibited significantly higher resistance to oxidative stressors such as menadione, H 2O2 and K2Cr2O7 than its astaxanthin-producing parental strain (MCP 324). The absence of carotenoids in the mutant did not explain this phenomenon. The cause of the decreased superoxide, hydroxyl radical and glutathione contents, the increased peroxide concentration and the elevated specific activity of catalase under uninduced conditions may be a second mutation. Peroxide treatment induced specific catalase activity in the mutant but not in the parental strain. Regulation of these processes led to the result that, in spite of the mutations, the two strains exhibited the same multiplication rate and generation time.

KW - carotenoid

KW - EPR

KW - GSH

KW - oxidative stress

KW - Phaffia rhodozyma

UR - http://www.scopus.com/inward/record.url?scp=79956088470&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=79956088470&partnerID=8YFLogxK

U2 - 10.1556/ABiol.62.2011.2.10

DO - 10.1556/ABiol.62.2011.2.10

M3 - Article

VL - 62

SP - 204

EP - 210

JO - Acta Biologica Hungarica

JF - Acta Biologica Hungarica

SN - 0236-5383

IS - 2

ER -