Characterization of rec15, an early meiotic recombination gene in Schizosaccharomyces pombe

Eveline Doll, M. Molnár, Yasushi Hiraoka, Jürg Kohli

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

In S. pombe strains mutant for rec15 aberrant ascus morphology, reduced spore viability and severe reduction of meiotic recombination was detected. Genetic and cytological analysis identified frequent interruption of meiosis after the first division, and nondisjunction I, as the main segregation errors in the mutant. Chromosome segregation at meiosis I was not random in rec15, suggesting the presence of a backup system for correct segregation of achiasmate chromosomes. The analysis of meiotic progression in time-course experiments revealed that the major meiotic events, such as the onset of premeiotic DNA synthesis, of horse-tail nuclear movement, and of the first meiotic division occurred earlier in rec15 than in wild-type. The early onset of meiotic events is a novel observation for an early recombination mutant and implies a function of rec15 protein already at or before DNA synthesis.

Original languageEnglish
Pages (from-to)323-333
Number of pages11
JournalCurrent Genetics
Volume48
Issue number5
DOIs
Publication statusPublished - Nov 2005

Fingerprint

Chromosome Segregation
Schizosaccharomyces
Meiosis
Genetic Recombination
DNA
Spores
Genes
Horses
Tail
Observation
Proteins

Keywords

  • Fission yeast
  • Meiosis
  • rec15
  • Recombination

ASJC Scopus subject areas

  • Genetics

Cite this

Characterization of rec15, an early meiotic recombination gene in Schizosaccharomyces pombe. / Doll, Eveline; Molnár, M.; Hiraoka, Yasushi; Kohli, Jürg.

In: Current Genetics, Vol. 48, No. 5, 11.2005, p. 323-333.

Research output: Contribution to journalArticle

Doll, Eveline ; Molnár, M. ; Hiraoka, Yasushi ; Kohli, Jürg. / Characterization of rec15, an early meiotic recombination gene in Schizosaccharomyces pombe. In: Current Genetics. 2005 ; Vol. 48, No. 5. pp. 323-333.
@article{5f02735fa1bc4088b2252b364aff2300,
title = "Characterization of rec15, an early meiotic recombination gene in Schizosaccharomyces pombe",
abstract = "In S. pombe strains mutant for rec15 aberrant ascus morphology, reduced spore viability and severe reduction of meiotic recombination was detected. Genetic and cytological analysis identified frequent interruption of meiosis after the first division, and nondisjunction I, as the main segregation errors in the mutant. Chromosome segregation at meiosis I was not random in rec15, suggesting the presence of a backup system for correct segregation of achiasmate chromosomes. The analysis of meiotic progression in time-course experiments revealed that the major meiotic events, such as the onset of premeiotic DNA synthesis, of horse-tail nuclear movement, and of the first meiotic division occurred earlier in rec15 than in wild-type. The early onset of meiotic events is a novel observation for an early recombination mutant and implies a function of rec15 protein already at or before DNA synthesis.",
keywords = "Fission yeast, Meiosis, rec15, Recombination",
author = "Eveline Doll and M. Moln{\'a}r and Yasushi Hiraoka and J{\"u}rg Kohli",
year = "2005",
month = "11",
doi = "10.1007/s00294-005-0024-3",
language = "English",
volume = "48",
pages = "323--333",
journal = "Current Genetics",
issn = "0172-8083",
publisher = "Springer Verlag",
number = "5",

}

TY - JOUR

T1 - Characterization of rec15, an early meiotic recombination gene in Schizosaccharomyces pombe

AU - Doll, Eveline

AU - Molnár, M.

AU - Hiraoka, Yasushi

AU - Kohli, Jürg

PY - 2005/11

Y1 - 2005/11

N2 - In S. pombe strains mutant for rec15 aberrant ascus morphology, reduced spore viability and severe reduction of meiotic recombination was detected. Genetic and cytological analysis identified frequent interruption of meiosis after the first division, and nondisjunction I, as the main segregation errors in the mutant. Chromosome segregation at meiosis I was not random in rec15, suggesting the presence of a backup system for correct segregation of achiasmate chromosomes. The analysis of meiotic progression in time-course experiments revealed that the major meiotic events, such as the onset of premeiotic DNA synthesis, of horse-tail nuclear movement, and of the first meiotic division occurred earlier in rec15 than in wild-type. The early onset of meiotic events is a novel observation for an early recombination mutant and implies a function of rec15 protein already at or before DNA synthesis.

AB - In S. pombe strains mutant for rec15 aberrant ascus morphology, reduced spore viability and severe reduction of meiotic recombination was detected. Genetic and cytological analysis identified frequent interruption of meiosis after the first division, and nondisjunction I, as the main segregation errors in the mutant. Chromosome segregation at meiosis I was not random in rec15, suggesting the presence of a backup system for correct segregation of achiasmate chromosomes. The analysis of meiotic progression in time-course experiments revealed that the major meiotic events, such as the onset of premeiotic DNA synthesis, of horse-tail nuclear movement, and of the first meiotic division occurred earlier in rec15 than in wild-type. The early onset of meiotic events is a novel observation for an early recombination mutant and implies a function of rec15 protein already at or before DNA synthesis.

KW - Fission yeast

KW - Meiosis

KW - rec15

KW - Recombination

UR - http://www.scopus.com/inward/record.url?scp=28044456955&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=28044456955&partnerID=8YFLogxK

U2 - 10.1007/s00294-005-0024-3

DO - 10.1007/s00294-005-0024-3

M3 - Article

VL - 48

SP - 323

EP - 333

JO - Current Genetics

JF - Current Genetics

SN - 0172-8083

IS - 5

ER -