Characterization of rapidly adhering amniotic fluid cells by combined immunofluorescence and phagocytosis assays

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Abstract

Culture of human amniotic-fluid cells from cases of fetal neural tube defects produces a population of rapidly adhering cells that were initially thought to be macrophages and later interpreted to be of neural origin. In this study double and triple labeling systems for the simultaneous detection of glial and macrophage differentiation marker antigens have been used to demonstrate that rapidly adhering cells cannot be considered a homogeneous population but instead represent two distinct cell types. One of these cell populations is of glial origin and shows specific staining for glial fibrillary acidic protein, while the other population is monocyte-derived macrophages which express marker antigens recognized by Leu M3, KiM7, and Dako antimacrophage monoclonal antibodies.

Original languageEnglish
Pages (from-to)786-792
Number of pages7
JournalAmerican Journal of Human Genetics
Volume45
Issue number5
Publication statusPublished - 1989

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Amniotic Fluid
Phagocytosis
Fluorescent Antibody Technique
Differentiation Antigens
Neuroglia
Population
Macrophages
Neural Tube Defects
Glial Fibrillary Acidic Protein
Monoclonal Antibodies
Staining and Labeling

ASJC Scopus subject areas

  • Genetics

Cite this

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abstract = "Culture of human amniotic-fluid cells from cases of fetal neural tube defects produces a population of rapidly adhering cells that were initially thought to be macrophages and later interpreted to be of neural origin. In this study double and triple labeling systems for the simultaneous detection of glial and macrophage differentiation marker antigens have been used to demonstrate that rapidly adhering cells cannot be considered a homogeneous population but instead represent two distinct cell types. One of these cell populations is of glial origin and shows specific staining for glial fibrillary acidic protein, while the other population is monocyte-derived macrophages which express marker antigens recognized by Leu M3, KiM7, and Dako antimacrophage monoclonal antibodies.",
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AU - Polgar, K.

AU - Ádány, R.

AU - Abel, G.

AU - Kappelmayer, J.

AU - Muszbek, L.

AU - Papp, Z.

PY - 1989

Y1 - 1989

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AB - Culture of human amniotic-fluid cells from cases of fetal neural tube defects produces a population of rapidly adhering cells that were initially thought to be macrophages and later interpreted to be of neural origin. In this study double and triple labeling systems for the simultaneous detection of glial and macrophage differentiation marker antigens have been used to demonstrate that rapidly adhering cells cannot be considered a homogeneous population but instead represent two distinct cell types. One of these cell populations is of glial origin and shows specific staining for glial fibrillary acidic protein, while the other population is monocyte-derived macrophages which express marker antigens recognized by Leu M3, KiM7, and Dako antimacrophage monoclonal antibodies.

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