Characterization of potential NMDA and cholecystokinin antagonists I. Acid-base properties of 2-methyl-4-oxo-3H-quinazoline-3-alkyl-carboxylic acids at the molecular and submolecular levels

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Abstract

The protonation macroconstants (logK) of 4(3H)-quinazolone (1) and two 2-methyl-4-oxo-3H-alkyl-quinazoline-3-carboxylic acid derivatives (2,3) were determined by pH-potentiometry. The acid-base chemistry of compounds 2 and 3, where proton-bindings take place in an overlapping fashion, was described in terms of protonation microconstants as well. Microspeciation was carried out by two means: UV-pH titration (selective, pH-dependent monitoring of the N1-binding site), and deductively (using a derivative compound as covalently fixed model of one of the protonation isomers). The microconstant values obtained by the two different methods are in agreement within 0.05 logK units. Microspeciation revealed remarkable differences between the two homologue compounds (2 and 3). The microconstant values show that insertion of a second methylene moiety into the aliphatic acid side-chain (1) increases the electron-density and most basicity parameters of both functional groups; (2) significantly modifies the extent of site-site interactions in the molecule; (3) opens new conformational preferences by N1 ring nitrogen-carboxylic group intramolecular hydrogen bond formation and (4) reverses the predominantly zwitterion-involved protonation pathway into a neutral form-involved pathway. These molecules exemplify that microconstant values allow the comparative prediction and quantitative evaluation of pharmacokinetic behaviour, and signify the fact that microspeciation is a powerful tool in the process of drug development. Copyright (C) 1999 Elsevier Science B.V.

Original languageEnglish
Pages (from-to)1-11
Number of pages11
JournalInternational Journal of Pharmaceutics
Volume180
Issue number1
DOIs
Publication statusPublished - Mar 25 1999

Keywords

  • Microspeciation
  • Protonation macro- and microconstants
  • Quinazolones

ASJC Scopus subject areas

  • Pharmaceutical Science

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