Characterization of polypyrrole/piperazine-1,4-bis(2-ethane sulfonate) film and its application for the immobilization of vitamin B12

Csaba Visy, Ivett Csízi, Emese Kriván

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3 Citations (Scopus)

Abstract

Vitamin B12 (B12) - possessing a redox active cobalt centre - is a candidate to be used as a mediator in bioelectrochemical processes. In order to exploit the possible redox activity of B12, Pt has been modified by a bio-conform conducting layer polypyrrole/piperazine-1,4-bis(2-ethane sulfonate) PPy/PIPES. The electrochemical and spectral behaviour of this film proved to be similar to PPy/dodecyl-sulfate (DS), widely considered and accepted as one of the best combinations of conducting polymer films. The capability of the PPy/PIPES film for acting as adsorbant in the accumulation of B12 has been evidenced by the electrochemical quartz crystal microbalance (EQCM) technique. B12 could also be incorporated into the polymer layer during its electrochemical deposition. The results proving the preserved redox activity of B12 within the film open new perspectives towards redox mediated bioelectrochemical applications based on the immobilization of this biomolecule.

Original languageEnglish
Pages (from-to)1189-1194
Number of pages6
JournalElectrochimica Acta
Volume53
Issue number3 SPEC. ISS.
DOIs
Publication statusPublished - Dec 20 2007

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Keywords

  • Biological buffer
  • Polypyrrole
  • Redox mediator
  • Vitamin B12

ASJC Scopus subject areas

  • Chemical Engineering(all)
  • Electrochemistry

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