Characterization of plant oils on a monolithic silica column by high-performance liquid chromatography-atmospheric pressure chemical ionization-mass spectrometry

A. Jakab, E. Forgács

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Abstract

Five plant oils (peanut, pumpkin seed, sesame seed, soybean, and wheat germ) have been analyzed by high-performance liquid chromatography-atmospheric pressure chemical ionization-mass spectrometry (HPLC-APCI-MS). Gradient elutian was performed with acetone-acetanitrile mobile phases an a short monolithic silica column (SilicaROD, RP-18e, 50mm × 4.6 mm). Identification of plant ail triacylglycerols (TAG) was based an the pseudomolecular ion [M + H]+ and the diacylglycerol [M - RCO2]+ fragments. Positional isomers of triacylglycerols were identified from the relative intensities of the [M - RCO2]+ fragments. Principal-component analysis, used to find similarities and differences between the different oils, indicated that the different plant oils could be clearly differentiated according to their triacylglycerol composition.

Original languageEnglish
Pages (from-to)S69-S73
JournalChromatographia
Volume56
Issue numberSUPPL.
Publication statusPublished - Jul 25 2002

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Keywords

  • LC-APCI-MS
  • Monolithic silica column
  • Plant oils
  • Principal-component analysis

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Clinical Biochemistry
  • Organic Chemistry

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