Characterization of plant oils on a monolithic silica column by high-performance liquid chromatography-atmospheric pressure chemical ionization-mass spectrometry

A. Jakab, E. Forgács

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Five plant oils (peanut, pumpkin seed, sesame seed, soybean, and wheat germ) have been analyzed by high-performance liquid chromatography-atmospheric pressure chemical ionization-mass spectrometry (HPLC-APCI-MS). Gradient elutian was performed with acetone-acetanitrile mobile phases an a short monolithic silica column (SilicaROD, RP-18e, 50mm × 4.6 mm). Identification of plant ail triacylglycerols (TAG) was based an the pseudomolecular ion [M + H]+ and the diacylglycerol [M - RCO2]+ fragments. Positional isomers of triacylglycerols were identified from the relative intensities of the [M - RCO2]+ fragments. Principal-component analysis, used to find similarities and differences between the different oils, indicated that the different plant oils could be clearly differentiated according to their triacylglycerol composition.

Original languageEnglish
JournalChromatographia
Volume56
Issue numberSUPPL.
Publication statusPublished - 2002

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Atmospheric Pressure
Plant Oils
High performance liquid chromatography
Silicon Dioxide
Atmospheric pressure
Ionization
Mass spectrometry
Mass Spectrometry
Triglycerides
High Pressure Liquid Chromatography
Seed
Seeds
Sesamum
Cucurbita
Diglycerides
Acetone
Principal Component Analysis
Soybeans
Isomers
Principal component analysis

Keywords

  • LC-APCI-MS
  • Monolithic silica column
  • Plant oils
  • Principal-component analysis

ASJC Scopus subject areas

  • Analytical Chemistry
  • Clinical Biochemistry

Cite this

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abstract = "Five plant oils (peanut, pumpkin seed, sesame seed, soybean, and wheat germ) have been analyzed by high-performance liquid chromatography-atmospheric pressure chemical ionization-mass spectrometry (HPLC-APCI-MS). Gradient elutian was performed with acetone-acetanitrile mobile phases an a short monolithic silica column (SilicaROD, RP-18e, 50mm × 4.6 mm). Identification of plant ail triacylglycerols (TAG) was based an the pseudomolecular ion [M + H]+ and the diacylglycerol [M - RCO2]+ fragments. Positional isomers of triacylglycerols were identified from the relative intensities of the [M - RCO2]+ fragments. Principal-component analysis, used to find similarities and differences between the different oils, indicated that the different plant oils could be clearly differentiated according to their triacylglycerol composition.",
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AU - Forgács, E.

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AB - Five plant oils (peanut, pumpkin seed, sesame seed, soybean, and wheat germ) have been analyzed by high-performance liquid chromatography-atmospheric pressure chemical ionization-mass spectrometry (HPLC-APCI-MS). Gradient elutian was performed with acetone-acetanitrile mobile phases an a short monolithic silica column (SilicaROD, RP-18e, 50mm × 4.6 mm). Identification of plant ail triacylglycerols (TAG) was based an the pseudomolecular ion [M + H]+ and the diacylglycerol [M - RCO2]+ fragments. Positional isomers of triacylglycerols were identified from the relative intensities of the [M - RCO2]+ fragments. Principal-component analysis, used to find similarities and differences between the different oils, indicated that the different plant oils could be clearly differentiated according to their triacylglycerol composition.

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KW - Monolithic silica column

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KW - Principal-component analysis

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