Characterization of photomorphogenic responses and signaling cascades controlled by phytochrome-A expressed in different tissues

Daniel Kirchenbauer, András Viczián, Éva Ádám, Zoltán Hegedűs, Cornelia Klose, Michael Leppert, Andreas Hiltbrunner, Stefan Kircher, Eberhard Schäfer, Ferenc Nagy

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12 Citations (Scopus)

Abstract

The photoreceptor phytochrome A acts as a light-dependent molecular switch and regulates responses initiated by very low fluences of light (VLFR) and high fluences (HIR) of far-red light. PhyA is expressed ubiquitously, but how phyA signaling is orchestrated to regulate photomorphogenesis is poorly understood. To address this issue, we generated transgenic Arabidopsis thaliana phyA-201 mutant lines expressing the biologically active phyA-YFP photoreceptor in different tissues, and analyzed the expression of several reporter genes, including ProHY5:HY5-GFP and Pro35S:CFP-PIF1, and various FR-HIR-dependent physiological responses. We show that phyA action in one tissue is critical and sufficient to regulate flowering time and root growth; control of cotyledon and hypocotyl growth requires simultaneous phyA activity in different tissues; and changes detected in the expression of reporters are not restricted to phyA-containing cells. We conclude that FR-HIR-controlled morphogenesis in Arabidopsis is mediated partly by tissue-specific and partly by intercellular signaling initiated by phyA. Intercellular signaling is critical for many FR-HIR induced responses, yet it appears that phyA modulates the abundance and activity of key regulatory transcription factors in a tissue-autonomous fashion.

Original languageEnglish
Pages (from-to)584-598
Number of pages15
JournalThe New phytologist
Volume211
Issue number2
DOIs
Publication statusPublished - Jul 1 2016

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Keywords

  • Arabidopsis thaliana
  • photomorphogenesis
  • phytochrome A
  • signaling
  • tissue/cell specificity
  • transcription

ASJC Scopus subject areas

  • Physiology
  • Plant Science

Cite this

Kirchenbauer, D., Viczián, A., Ádám, É., Hegedűs, Z., Klose, C., Leppert, M., Hiltbrunner, A., Kircher, S., Schäfer, E., & Nagy, F. (2016). Characterization of photomorphogenic responses and signaling cascades controlled by phytochrome-A expressed in different tissues. The New phytologist, 211(2), 584-598. https://doi.org/10.1111/nph.13941