Characterization of photoautotrophic picoplankton assemblages in turbid, alkaline lakes of the Carpathian Basin (Central Europe)

Tamás Felföldi, Boglárka Somogyi, K. Márialigeti, Lajos Vörös

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

The photoautotrophic picoplankton (PPP) of ten shallow, hyposaline soda lakes located in three different geographical regions in the Carpathian Basin (Central Europe) was characterized. These lakes, which frequently dry out completely, are extremely rich in PPP. Epifluorescence microscopy was applied to determine picocyanobacterial and picoeukaryotic cell abundance and PCR-based molecular techniques (denaturing gradient gel electrophoresis and cloning with phylospecies delineation) to identify the members of PPP. Most of these lakes were eu- and hypertrophic with varying contribution of picocyanobacteria to the total PPP cell number. We found an unusually high PPP abundance with peaks of 8.16 × 106 cells mL-1 for picoeukaryotes and 1.78 × 107 cells mL-1 for picocyanobacteria. The majority of the retrieved PPP sequences belonged to picocyanobacteria (nonmarine Synechococcus/ Cyanobium), while others showed similarity to eukaryotic algal plastids (close to Trebouxiophycean isolates). Molecular analysis revealed significant genetic diversity in the PPP fraction of these lakes and showed that the closest relatives of our picocyanobacterial clones were recovered from different habitats, indicating seemingly no correlation between the 'saline' ecotypes and their phylogenetic position. Our results also confirmed that PPP might exploit different aquatic ecosystems and be successful even in the case of abrupt changes of environmental parameters (in our case, salinity). According to our knowledge, this is the first survey focusing on the identification of the PPP community members in turbid and alkaline lakes with extraordinarily high picoplankton productivity.

Original languageEnglish
Pages (from-to)385-395
Number of pages11
JournalJournal of Limnology
Volume68
Issue number2
DOIs
Publication statusPublished - 2009

Fingerprint

picoplankton
Central European region
basins
lakes
lake
basin
cells
Synechococcus
denaturing gradient gel electrophoresis
ecotypes
plastids
molecular cloning
microscopy
Europe
clones
salinity
genetic variation
molecular analysis
ecotype
phylogeny

Keywords

  • Epifluorescence microscopy
  • PCR-based molecular techniques
  • Photoautotrophic picoplankton
  • Soda lake

ASJC Scopus subject areas

  • Water Science and Technology
  • Aquatic Science
  • Ecology

Cite this

Characterization of photoautotrophic picoplankton assemblages in turbid, alkaline lakes of the Carpathian Basin (Central Europe). / Felföldi, Tamás; Somogyi, Boglárka; Márialigeti, K.; Vörös, Lajos.

In: Journal of Limnology, Vol. 68, No. 2, 2009, p. 385-395.

Research output: Contribution to journalArticle

@article{feb8195745bc412e921d91c412dec05d,
title = "Characterization of photoautotrophic picoplankton assemblages in turbid, alkaline lakes of the Carpathian Basin (Central Europe)",
abstract = "The photoautotrophic picoplankton (PPP) of ten shallow, hyposaline soda lakes located in three different geographical regions in the Carpathian Basin (Central Europe) was characterized. These lakes, which frequently dry out completely, are extremely rich in PPP. Epifluorescence microscopy was applied to determine picocyanobacterial and picoeukaryotic cell abundance and PCR-based molecular techniques (denaturing gradient gel electrophoresis and cloning with phylospecies delineation) to identify the members of PPP. Most of these lakes were eu- and hypertrophic with varying contribution of picocyanobacteria to the total PPP cell number. We found an unusually high PPP abundance with peaks of 8.16 × 106 cells mL-1 for picoeukaryotes and 1.78 × 107 cells mL-1 for picocyanobacteria. The majority of the retrieved PPP sequences belonged to picocyanobacteria (nonmarine Synechococcus/ Cyanobium), while others showed similarity to eukaryotic algal plastids (close to Trebouxiophycean isolates). Molecular analysis revealed significant genetic diversity in the PPP fraction of these lakes and showed that the closest relatives of our picocyanobacterial clones were recovered from different habitats, indicating seemingly no correlation between the 'saline' ecotypes and their phylogenetic position. Our results also confirmed that PPP might exploit different aquatic ecosystems and be successful even in the case of abrupt changes of environmental parameters (in our case, salinity). According to our knowledge, this is the first survey focusing on the identification of the PPP community members in turbid and alkaline lakes with extraordinarily high picoplankton productivity.",
keywords = "Epifluorescence microscopy, PCR-based molecular techniques, Photoautotrophic picoplankton, Soda lake",
author = "Tam{\'a}s Felf{\"o}ldi and Bogl{\'a}rka Somogyi and K. M{\'a}rialigeti and Lajos V{\"o}r{\"o}s",
year = "2009",
doi = "10.3274/JL09-68-2-21",
language = "English",
volume = "68",
pages = "385--395",
journal = "Journal of Limnology",
issn = "1129-5767",
publisher = "PagePress",
number = "2",

}

TY - JOUR

T1 - Characterization of photoautotrophic picoplankton assemblages in turbid, alkaline lakes of the Carpathian Basin (Central Europe)

AU - Felföldi, Tamás

AU - Somogyi, Boglárka

AU - Márialigeti, K.

AU - Vörös, Lajos

PY - 2009

Y1 - 2009

N2 - The photoautotrophic picoplankton (PPP) of ten shallow, hyposaline soda lakes located in three different geographical regions in the Carpathian Basin (Central Europe) was characterized. These lakes, which frequently dry out completely, are extremely rich in PPP. Epifluorescence microscopy was applied to determine picocyanobacterial and picoeukaryotic cell abundance and PCR-based molecular techniques (denaturing gradient gel electrophoresis and cloning with phylospecies delineation) to identify the members of PPP. Most of these lakes were eu- and hypertrophic with varying contribution of picocyanobacteria to the total PPP cell number. We found an unusually high PPP abundance with peaks of 8.16 × 106 cells mL-1 for picoeukaryotes and 1.78 × 107 cells mL-1 for picocyanobacteria. The majority of the retrieved PPP sequences belonged to picocyanobacteria (nonmarine Synechococcus/ Cyanobium), while others showed similarity to eukaryotic algal plastids (close to Trebouxiophycean isolates). Molecular analysis revealed significant genetic diversity in the PPP fraction of these lakes and showed that the closest relatives of our picocyanobacterial clones were recovered from different habitats, indicating seemingly no correlation between the 'saline' ecotypes and their phylogenetic position. Our results also confirmed that PPP might exploit different aquatic ecosystems and be successful even in the case of abrupt changes of environmental parameters (in our case, salinity). According to our knowledge, this is the first survey focusing on the identification of the PPP community members in turbid and alkaline lakes with extraordinarily high picoplankton productivity.

AB - The photoautotrophic picoplankton (PPP) of ten shallow, hyposaline soda lakes located in three different geographical regions in the Carpathian Basin (Central Europe) was characterized. These lakes, which frequently dry out completely, are extremely rich in PPP. Epifluorescence microscopy was applied to determine picocyanobacterial and picoeukaryotic cell abundance and PCR-based molecular techniques (denaturing gradient gel electrophoresis and cloning with phylospecies delineation) to identify the members of PPP. Most of these lakes were eu- and hypertrophic with varying contribution of picocyanobacteria to the total PPP cell number. We found an unusually high PPP abundance with peaks of 8.16 × 106 cells mL-1 for picoeukaryotes and 1.78 × 107 cells mL-1 for picocyanobacteria. The majority of the retrieved PPP sequences belonged to picocyanobacteria (nonmarine Synechococcus/ Cyanobium), while others showed similarity to eukaryotic algal plastids (close to Trebouxiophycean isolates). Molecular analysis revealed significant genetic diversity in the PPP fraction of these lakes and showed that the closest relatives of our picocyanobacterial clones were recovered from different habitats, indicating seemingly no correlation between the 'saline' ecotypes and their phylogenetic position. Our results also confirmed that PPP might exploit different aquatic ecosystems and be successful even in the case of abrupt changes of environmental parameters (in our case, salinity). According to our knowledge, this is the first survey focusing on the identification of the PPP community members in turbid and alkaline lakes with extraordinarily high picoplankton productivity.

KW - Epifluorescence microscopy

KW - PCR-based molecular techniques

KW - Photoautotrophic picoplankton

KW - Soda lake

UR - http://www.scopus.com/inward/record.url?scp=69949095095&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=69949095095&partnerID=8YFLogxK

U2 - 10.3274/JL09-68-2-21

DO - 10.3274/JL09-68-2-21

M3 - Article

AN - SCOPUS:69949095095

VL - 68

SP - 385

EP - 395

JO - Journal of Limnology

JF - Journal of Limnology

SN - 1129-5767

IS - 2

ER -