Characterization of Pasteurella multocida strains isolated from human infections

B. Ujvári, R. Weiczner, Z. Deim, G. Terhes, E. Urbán, A. R. Tóth, T. Magyar

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Isolates of Pasteurella multocida recovered from infected humans (n = 15) were characterized by traditional and molecular microbiological methods and were compared with cat-derived strains (n = 5). The most prevalent subspecies among strains from human infections was P. multocida subsp. septica (80%), and nearly all isolates showed a similar combination of virulence-associated genes. MLST analysis classified the 20 P. multocida strains into 16 different sequence types, and we assigned 11 new sequence types (ST), however, only one of those (ST 334) was shared by two human and one cat isolates. P. multocida subsp. septica strains formed a distinct phylogenetic group within the species. The strains showed resistance to erythromycin, clindamycin and sulfamethoxazole, and with two exceptions, resistance to tilmicosin was also detected. Each strain was susceptible to ampicillin, streptomycin, gentamycin, tetracycline, doxycycline, cefazolin, cefpodoxime, chloramphenicol, florfenicol and enrofloxacin. Common characteristics (virulence profile and antibiotic sensitivity pattern) shared by strains isolated from humans and cats support the view that domestic cats may serve as a potential reservoir for P. multocida.

Original languageEnglish
Pages (from-to)37-43
Number of pages7
JournalComparative Immunology, Microbiology and Infectious Diseases
Volume63
DOIs
Publication statusPublished - Apr 1 2019

Fingerprint

Pasteurella multocida
Cats
Pasteurella multocida subsp. septica
Infection
infection
cefpodoxime
cats
Virulence
Sulfamethoxazole
Cefazolin
Clindamycin
Doxycycline
virulence
Streptomycin
Chloramphenicol
Erythromycin
Ampicillin
cefazolin
Gentamicins
Tetracycline

Keywords

  • Antimicrobial resistance
  • Human infections
  • Multilocus sequence typing
  • Pasteurella multocida

ASJC Scopus subject areas

  • Microbiology
  • Immunology and Allergy
  • Immunology
  • veterinary(all)
  • Infectious Diseases

Cite this

Characterization of Pasteurella multocida strains isolated from human infections. / Ujvári, B.; Weiczner, R.; Deim, Z.; Terhes, G.; Urbán, E.; Tóth, A. R.; Magyar, T.

In: Comparative Immunology, Microbiology and Infectious Diseases, Vol. 63, 01.04.2019, p. 37-43.

Research output: Contribution to journalArticle

Ujvári, B. ; Weiczner, R. ; Deim, Z. ; Terhes, G. ; Urbán, E. ; Tóth, A. R. ; Magyar, T. / Characterization of Pasteurella multocida strains isolated from human infections. In: Comparative Immunology, Microbiology and Infectious Diseases. 2019 ; Vol. 63. pp. 37-43.
@article{4b6e37ded4e74f70a7695f5228be4ba0,
title = "Characterization of Pasteurella multocida strains isolated from human infections",
abstract = "Isolates of Pasteurella multocida recovered from infected humans (n = 15) were characterized by traditional and molecular microbiological methods and were compared with cat-derived strains (n = 5). The most prevalent subspecies among strains from human infections was P. multocida subsp. septica (80{\%}), and nearly all isolates showed a similar combination of virulence-associated genes. MLST analysis classified the 20 P. multocida strains into 16 different sequence types, and we assigned 11 new sequence types (ST), however, only one of those (ST 334) was shared by two human and one cat isolates. P. multocida subsp. septica strains formed a distinct phylogenetic group within the species. The strains showed resistance to erythromycin, clindamycin and sulfamethoxazole, and with two exceptions, resistance to tilmicosin was also detected. Each strain was susceptible to ampicillin, streptomycin, gentamycin, tetracycline, doxycycline, cefazolin, cefpodoxime, chloramphenicol, florfenicol and enrofloxacin. Common characteristics (virulence profile and antibiotic sensitivity pattern) shared by strains isolated from humans and cats support the view that domestic cats may serve as a potential reservoir for P. multocida.",
keywords = "Antimicrobial resistance, Human infections, Multilocus sequence typing, Pasteurella multocida",
author = "B. Ujv{\'a}ri and R. Weiczner and Z. Deim and G. Terhes and E. Urb{\'a}n and T{\'o}th, {A. R.} and T. Magyar",
year = "2019",
month = "4",
day = "1",
doi = "10.1016/j.cimid.2018.12.008",
language = "English",
volume = "63",
pages = "37--43",
journal = "Comparative Immunology, Microbiology and Infectious Diseases",
issn = "0147-9571",
publisher = "Elsevier Limited",

}

TY - JOUR

T1 - Characterization of Pasteurella multocida strains isolated from human infections

AU - Ujvári, B.

AU - Weiczner, R.

AU - Deim, Z.

AU - Terhes, G.

AU - Urbán, E.

AU - Tóth, A. R.

AU - Magyar, T.

PY - 2019/4/1

Y1 - 2019/4/1

N2 - Isolates of Pasteurella multocida recovered from infected humans (n = 15) were characterized by traditional and molecular microbiological methods and were compared with cat-derived strains (n = 5). The most prevalent subspecies among strains from human infections was P. multocida subsp. septica (80%), and nearly all isolates showed a similar combination of virulence-associated genes. MLST analysis classified the 20 P. multocida strains into 16 different sequence types, and we assigned 11 new sequence types (ST), however, only one of those (ST 334) was shared by two human and one cat isolates. P. multocida subsp. septica strains formed a distinct phylogenetic group within the species. The strains showed resistance to erythromycin, clindamycin and sulfamethoxazole, and with two exceptions, resistance to tilmicosin was also detected. Each strain was susceptible to ampicillin, streptomycin, gentamycin, tetracycline, doxycycline, cefazolin, cefpodoxime, chloramphenicol, florfenicol and enrofloxacin. Common characteristics (virulence profile and antibiotic sensitivity pattern) shared by strains isolated from humans and cats support the view that domestic cats may serve as a potential reservoir for P. multocida.

AB - Isolates of Pasteurella multocida recovered from infected humans (n = 15) were characterized by traditional and molecular microbiological methods and were compared with cat-derived strains (n = 5). The most prevalent subspecies among strains from human infections was P. multocida subsp. septica (80%), and nearly all isolates showed a similar combination of virulence-associated genes. MLST analysis classified the 20 P. multocida strains into 16 different sequence types, and we assigned 11 new sequence types (ST), however, only one of those (ST 334) was shared by two human and one cat isolates. P. multocida subsp. septica strains formed a distinct phylogenetic group within the species. The strains showed resistance to erythromycin, clindamycin and sulfamethoxazole, and with two exceptions, resistance to tilmicosin was also detected. Each strain was susceptible to ampicillin, streptomycin, gentamycin, tetracycline, doxycycline, cefazolin, cefpodoxime, chloramphenicol, florfenicol and enrofloxacin. Common characteristics (virulence profile and antibiotic sensitivity pattern) shared by strains isolated from humans and cats support the view that domestic cats may serve as a potential reservoir for P. multocida.

KW - Antimicrobial resistance

KW - Human infections

KW - Multilocus sequence typing

KW - Pasteurella multocida

UR - http://www.scopus.com/inward/record.url?scp=85059645734&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85059645734&partnerID=8YFLogxK

U2 - 10.1016/j.cimid.2018.12.008

DO - 10.1016/j.cimid.2018.12.008

M3 - Article

C2 - 30961816

AN - SCOPUS:85059645734

VL - 63

SP - 37

EP - 43

JO - Comparative Immunology, Microbiology and Infectious Diseases

JF - Comparative Immunology, Microbiology and Infectious Diseases

SN - 0147-9571

ER -