Characterization of Pasteurella multocida strains isolated from geese

Zsuzsanna Varga, Dmitriy V. Volokhov, L. Stipkovits, ákos Thuma, Boglárka Sellyei, Tibor Magyar

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Phenotypic and genotypic diversity of forty-two Pasteurella multocida isolates from geese were characterized by analysis of their capsular type, Heddleston serotype, biotype, fimbrial gene allele type, comparative outer membrane protein (OMP) electrophoresis patterns, and were analyzed using PCR for the presence of virulence-associated genes (. toxA, tbpA, pfhA, hgbA, hgbB, nanH, nanB, fimA, hsf-1, and pmHAS). A sequence comparison of the thdF and rpoB housekeeping genes of twenty representative P. multocida strains from three different OMP groups demonstrated that seventeen strains were closely related phylogenetically to previously published strains of P. multocida subsp. multocida and P. multocida subsp. gallicida, and only three strains from geese were grouped with previously published strains of P. multocida subsp. septica. This study is the first report regarding the characterization of phenotypic and genotypic features of different P. multocida field strains isolated from geese with the different clinical representation of pasteurellosis and provide our overview on the usefulness of these in vitro tests for primary characterization of P. multocida strains for the epidemiological studies of fowl cholera.

Original languageEnglish
Pages (from-to)149-156
Number of pages8
JournalVeterinary Microbiology
Volume163
Issue number1-2
DOIs
Publication statusPublished - 2013

Fingerprint

Geese
Pasteurella multocida
geese
outer membrane proteins
Pasteurella multocida subsp. gallicida
Pasteurella multocida subsp. septica
Pasteurella multocida subsp. multocida
Membrane Proteins
Pasteurella Infections
fowl cholera
pasteurellosis
Cholera
Essential Genes
biotypes
epidemiological studies
Genes
electrophoresis
Virulence
Electrophoresis
Epidemiologic Studies

Keywords

  • Fowl cholera
  • Goose
  • Pasteurella multocida
  • Pasteurellosis

ASJC Scopus subject areas

  • Microbiology
  • veterinary(all)

Cite this

Characterization of Pasteurella multocida strains isolated from geese. / Varga, Zsuzsanna; Volokhov, Dmitriy V.; Stipkovits, L.; Thuma, ákos; Sellyei, Boglárka; Magyar, Tibor.

In: Veterinary Microbiology, Vol. 163, No. 1-2, 2013, p. 149-156.

Research output: Contribution to journalArticle

Varga, Z, Volokhov, DV, Stipkovits, L, Thuma, Á, Sellyei, B & Magyar, T 2013, 'Characterization of Pasteurella multocida strains isolated from geese', Veterinary Microbiology, vol. 163, no. 1-2, pp. 149-156. https://doi.org/10.1016/j.vetmic.2012.12.023
Varga, Zsuzsanna ; Volokhov, Dmitriy V. ; Stipkovits, L. ; Thuma, ákos ; Sellyei, Boglárka ; Magyar, Tibor. / Characterization of Pasteurella multocida strains isolated from geese. In: Veterinary Microbiology. 2013 ; Vol. 163, No. 1-2. pp. 149-156.
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