Characterization of ouabain-like immunoreactivity in human urine

Stefan Worgall, Joerg Hänze, Roswitha Wagner, Christian Peiser, Rudolf E. Lang, Endre Sulyok, Wolfgang Rascher

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Abstract

Objective: To characterize ouabain-like immunoreactivity in human urine. Methods: Sensitive radioimmunoassay for ouabain characterized by high-peformance liquid chromatography. Results: Serial dilution of urinary immunoreactive ouabain paralleled the standard curve, but not so plasma immunoreactive ouabain. Intravenous administration of 86 nmol (62.5 μg) ouabain caused a rapid rise in ouabain immunoreactivity in plasma of healthy volunteers with a maximum of 1.7 nmol/l 8 min after injection and returned to basal levels after 6 h. Ouabain immunoreactivity rose to 36 nmol/l in urine, suggesting that exogenously administered ouabain can be measured reliably in plasma and urine. Analytical reversed-phase high-performance liquid chromatography (isopropanol-propanol biphasic gradient; linear acetonitrile gradient) of sample extracts before assay demonstrated measurable amounts of ouabain-related material only in native urine, but not in plasma. When plasma and urine were spiked with ouabain standard or normal volunteers were injected with ouabain, the assay reliably measured ouabain. Conclusion: A substance closely related to ouabain can be detected in urine, but circulates, if at all, in small amounts in human plasma.

Original languageEnglish
Pages (from-to)623-628
Number of pages6
JournalJournal of hypertension
Volume14
Issue number5
DOIs
Publication statusPublished - Jun 7 1996

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Keywords

  • Essential hypertension
  • High-performance liquid chromatography
  • Ouabain
  • Radioimmunoassay
  • Sodium

ASJC Scopus subject areas

  • Internal Medicine
  • Physiology
  • Cardiology and Cardiovascular Medicine

Cite this

Worgall, S., Hänze, J., Wagner, R., Peiser, C., Lang, R. E., Sulyok, E., & Rascher, W. (1996). Characterization of ouabain-like immunoreactivity in human urine. Journal of hypertension, 14(5), 623-628. https://doi.org/10.1097/00004872-199605000-00012