Characterization of novel transcripts in pseudorabies virus

Dora Tombacz, Zsolt Csabai, Péter Olâh, Zoltan Havelda, Donald Sharon, Michael Snyder, Zsolt Boldogkôi

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

In this study we identified two 3'-coterminal RNA molecules in the pseudorabies virus. The highly abundant short transcript (CTO-S) proved to be encoded between the ul21 and ul22 genes in close vicinity of the replication origin (OriL) of the virus. The less abundant long RNA molecule (CTO-L) is a transcriptional readthrough product of the ul21 gene and overlaps OriL. These polyadenylated RNAs were characterized by ascertaining their nucleotide sequences with the Illumina HiScanSQ and Pacific Biosciences Real-Time (PacBio RSII) sequencing platforms and by analyzing their transcription kinetics through use of multi-time-point Real-Time RT-PCR and the PacBio RSII system. It emerged that transcription of the CTOs is fully dependent on the viral transactivator protein IE180 and CTO-S is not a microRNA precursor. We propose an interaction between the transcription and replication machineries at this genomic location, which might play an important role in the regulation of DNA synthesis.

Original languageEnglish
Pages (from-to)2727-2744
Number of pages18
JournalViruses
Volume7
Issue number5
DOIs
Publication statusPublished - May 22 2015

Keywords

  • DNA replication
  • Herpesvirus
  • Non-coding RNA
  • Pseudorabies virus
  • RNA sequencing

ASJC Scopus subject areas

  • Infectious Diseases
  • Virology

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  • Cite this

    Tombacz, D., Csabai, Z., Olâh, P., Havelda, Z., Sharon, D., Snyder, M., & Boldogkôi, Z. (2015). Characterization of novel transcripts in pseudorabies virus. Viruses, 7(5), 2727-2744. https://doi.org/10.3390/v7052727