Characterization of neurons in the cortical white matter in human temporal lobe epilepsy

Zsófia Richter, J. Janszky, György Sétáló, Réka Horváth, Zsolt Horváth, T. Dóczi, L. Séress, H. Ábrahám

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

The aim of the present work was to characterize neurons in the archi- and neocortical white matter, and to investigate their distribution in mesial temporal sclerosis. Immunohistochemistry and quantification of neurons were performed on surgically resected tissue sections of patients with therapy-resistant temporal lobe epilepsy. Temporal lobe tissues of patients with tumor but without epilepsy and that from autopsy were used as controls. Neurons were identified with immunohistochemistry using antibodies against NeuN, calcium-binding proteins, transcription factor Tbr1 and neurofilaments. We found significantly higher density of neurons in the archi- and neocortical white matter of patients with temporal lobe epilepsy than in that of controls. Based on their morphology and neurochemical content, both excitatory and inhibitory cells were present among these neurons. A subset of neurons in the white matter was Tbr-1-immunoreactive and these neurons coexpressed NeuN and neurofilament marker SMI311R. No colocalization of Tbr1 was observed with the inhibitory neuronal markers, calcium-binding proteins. We suggest that a large population of white matter neurons comprises remnants of the subplate. Furthermore, we propose that a subset of white matter neurons was arrested during migration, highlighting the role of cortical maldevelopment in epilepsy associated with mesial temporal sclerosis.

Original languageEnglish
Pages (from-to)140-150
Number of pages11
JournalNeuroscience
Volume333
DOIs
Publication statusPublished - Oct 1 2016

Fingerprint

Temporal Lobe Epilepsy
Neurons
Calcium-Binding Proteins
Intermediate Filaments
Sclerosis
Epilepsy
Immunohistochemistry
White Matter
Temporal Lobe
Autopsy
Transcription Factors
Antibodies

Keywords

  • epileptic seizures
  • excitatory neurons
  • inhibitory neurons
  • neuronal network
  • subplate

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Characterization of neurons in the cortical white matter in human temporal lobe epilepsy. / Richter, Zsófia; Janszky, J.; Sétáló, György; Horváth, Réka; Horváth, Zsolt; Dóczi, T.; Séress, L.; Ábrahám, H.

In: Neuroscience, Vol. 333, 01.10.2016, p. 140-150.

Research output: Contribution to journalArticle

Richter, Zsófia ; Janszky, J. ; Sétáló, György ; Horváth, Réka ; Horváth, Zsolt ; Dóczi, T. ; Séress, L. ; Ábrahám, H. / Characterization of neurons in the cortical white matter in human temporal lobe epilepsy. In: Neuroscience. 2016 ; Vol. 333. pp. 140-150.
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